Overview

  • Product name

    Anti-ATPB antibody [3D5] - Mitochondrial Marker (HRP)
    See all ATPB primary antibodies
  • Description

    Mouse monoclonal [3D5] to ATPB - Mitochondrial Marker (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Caenorhabditis elegans, Monkey
  • Immunogen

    Tissue, cells or virus corresponding to Human ATPB.

  • Positive control

    • WB: Human heart mitochondrial lysate. HeLa and HepG2 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab197905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Target

  • Function

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • Sequence similarities

    Belongs to the ATPase alpha/beta chains family.
  • Cellular localization

    Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP 5B antibody
    • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
    • ATP synthase subunit beta mitochondrial antibody
    • ATP synthase subunit beta, mitochondrial antibody
    • atp5b antibody
    • ATPB antibody
    • ATPB_HUMAN antibody
    • ATPMB antibody
    • ATPSB antibody
    • Epididymis secretory protein Li 271 antibody
    • HEL-S-271 antibody
    • Mitochondrial ATP synthase beta subunit antibody
    • Mitochondrial ATP Synthase Subunit Beta antibody
    • Mitochondrial ATP synthetase beta subunit antibody
    see all

Images

  • All lanes : Anti-ATPB antibody [3D5] - Mitochondrial Marker (HRP) (ab197905) at 1/5000 dilution

    Lane 1 : Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg
    Lane 2 : HeLa whole cell lysate (ab150035) at 10 µg
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa


    Exposure time: 2 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197905 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab197905 has not yet been referenced specifically in any publications.

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