Product nameAnti-ATPB antibody [4.3E8.D10]
See all ATPB primary antibodies
DescriptionMouse monoclonal [4.3E8.D10] to ATPB
SpecificityDetects the beta subunit of ATP synthase (ATPB) from mouse rat and human samples. This antibody is useful as a mitochondrial marker.
Tested applicationsSuitable for: WB, ICC, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Other Immunogen Type corresponding to ATPB. Intact rat mitochondria.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab5432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).|
|ICC||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
|IP||Use a concentration of 2 - 5 µg/ml.
By immunoprecipatation, this antibody detects an 50 kDa protein representing ATP synthase (ATPB) from solubilized rat brain mitochondria.
FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Sequence similaritiesBelongs to the ATPase alpha/beta chains family.
Cellular localizationMitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
- Information by UniProt
- ATP 5B antibody
- ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
- ATP synthase subunit beta mitochondrial antibody
Anti-ATPB antibody [4.3E8.D10] (ab5432) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 20 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 16 minutes
Immunocytochemistry/Immunofluorescent analysis of ATPB (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with ab5432 at 1:100 overnight at 4ºC in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification.
Immunofluorescent analysis of ATPB in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATPB monoclonal antibody (ab5432) at a dilution of 1:200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. ATPB staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Immunofluorescent analysis of ATPB in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATPB monoclonal antibody (ab5432) at a dilution of 1:200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. ATPB staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Immunoprecipatation of rat neuronal/glial cell extract using ab5432.
ICC/IF image of ab5432 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/Immunofluorescence analysis of rat neuronal/glial cell culture using ab5432.
This product has been referenced in:
- Diokmetzidou A et al. Desmin and aB-crystallin interplay in the maintenance of mitochondrial homeostasis and cardiomyocyte survival. J Cell Sci 129:3705-3720 (2016). Read more (PubMed: 27566162) »
- Laker RC et al. The Mitochondrial Permeability Transition Pore Regulator Cyclophilin D Exhibits Tissue-Specific Control of Metabolic Homeostasis. PLoS One 11:e0167910 (2016). WB . Read more (PubMed: 28005946) »