Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
Key features and details
- Mouse monoclonal [4.3E8.D10] to ATPB - Mitochondrial Marker
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker
See all ATPB primary antibodies -
Description
Mouse monoclonal [4.3E8.D10] to ATPB - Mitochondrial Marker -
Host species
Mouse -
Specificity
Detects the beta subunit of ATP synthase (ATPB) from mouse rat and human samples. This antibody is useful as a mitochondrial marker. -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Tissue, cells or virus corresponding to ATPB. Intact rat mitochondria.
-
Positive control
- ICC/IF: 3T3, HEK293T, A431, rat neuronal glial cells, HeLa; IP: rat neuronal/glial cells, THP-1 cells; WB: human testis tissue lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
4.3E8.D10 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
|
ICC/IF |
1/100 - 1/1000.
|
|
IP |
Use a concentration of 2 - 5 µg/ml.
By immunoprecipatation, this antibody detects an 50 kDa protein representing ATP synthase (ATPB) from solubilized rat brain mitochondria. |
Notes |
---|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
ICC/IF
1/100 - 1/1000. |
IP
Use a concentration of 2 - 5 µg/ml. By immunoprecipatation, this antibody detects an 50 kDa protein representing ATP synthase (ATPB) from solubilized rat brain mitochondria. |
Target
-
Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. -
Sequence similarities
Belongs to the ATPase alpha/beta chains family. -
Cellular localization
Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein. - Information by UniProt
-
Database links
- Entrez Gene: 506 Human
- Entrez Gene: 11947 Mouse
- Entrez Gene: 171374 Rat
- GenBank: BC016512 Human
- Omim: 102910 Human
- SwissProt: P06576 Human
- SwissProt: P56480 Mouse
- SwissProt: P10719 Rat
see all -
Alternative names
- ATP 5B antibody
- ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
- ATP synthase subunit beta mitochondrial antibody
see all
Images
-
Immunocytochemistry analysis of ATPB using ab5432 at 5µg/mL concentration shows staining in 4% paraformaldehyde-fixed 3T3 Cells. Secondary was Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at 1/1000 dilution. ATPB (green), and nuclei with Hoechst 33342 dye (blue) is shown. Negative control has no primary antibody
-
ATPB was immunoprecipitated from THP-1 whole cell lysate with 5 µL ab5432.Lane 1: ab5432 IP in THP-1 whole cell lysate, with HRP-conjugated goat anti-mouse IgG secondary
Detection: Chemiluminescence
-
Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 20 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 16 minutes -
Immunocytochemistry/Immunofluorescent analysis of ATPB (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with ab5432 at 1:100 overnight at 4ºC in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification.
-
Immunofluorescent analysis of ATPB in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATPB monoclonal antibody (ab5432) at a dilution of 1:200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. ATPB staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
-
Immunofluorescent analysis of ATPB in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATPB monoclonal antibody (ab5432) at a dilution of 1:200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. ATPB staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
-
Immunoprecipatation of rat neuronal/glial cell extract using ab5432.
-
ICC/IF image of ab5432 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunocytochemistry/Immunofluorescence analysis of rat neuronal/glial cell culture using ab5432.
Protocols
Datasheets and documents
-
Datasheet download
References (19)
ab5432 has been referenced in 19 publications.
- Ostersehlt LM et al. DNA-PAINT MINFLUX nanoscopy. Nat Methods 19:1072-1075 (2022). PubMed: 36050490
- Ortin-Martinez A et al. Photoreceptor nanotubes mediate the in vivo exchange of intracellular material. EMBO J 40:e107264 (2021). PubMed: 34494680
- Tameling C et al. Colocalization for super-resolution microscopy via optimal transport. Nat Comput Sci 1:199-211 (2021). PubMed: 35874932
- Steinberg G et al. A lipophilic cation protects crops against fungal pathogens by multiple modes of action. Nat Commun 11:1608 (2020). PubMed: 32231209
- Rumyantseva A et al. DARS2 is indispensable for Purkinje cell survival and protects against cerebellar ataxia. Hum Mol Genet 29:2845-2854 (2020). PubMed: 32766765