Overview

  • Product name

    Anti-ATPB antibody [6C10]
    See all ATPB primary antibodies
  • Description

    Mouse monoclonal [6C10] to ATPB
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Dog, Human
    Predicted to work with: Mouse, Chicken, Cow
  • Immunogen

    Recombinant full length protein corresponding to Human ATPB. NP_001677. Produced in HEK-293T cells.
    Database link: P06576

  • Positive control

    • IHC-P: Human skeletal muscle tissue. WB: pCMV6-ENTRY ATPB transfected HEK-293T cell lysate. HeLa, HepG2, MDCK and PC-12 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab230706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 57 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • Sequence similarities

    Belongs to the ATPase alpha/beta chains family.
  • Cellular localization

    Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP 5B antibody
    • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
    • ATP synthase subunit beta mitochondrial antibody
    • ATP synthase subunit beta, mitochondrial antibody
    • atp5b antibody
    • ATPB antibody
    • ATPB_HUMAN antibody
    • ATPMB antibody
    • ATPSB antibody
    • Epididymis secretory protein Li 271 antibody
    • HEL-S-271 antibody
    • Mitochondrial ATP synthase beta subunit antibody
    • Mitochondrial ATP Synthase Subunit Beta antibody
    • Mitochondrial ATP synthetase beta subunit antibody
    see all

Images

  • All lanes : Anti-ATPB antibody [6C10] (ab230706) at 1/2000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extracts
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
    Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) cell extracts
    Lane 4 : A549 (Human lung carcinoma cell line) cell extracts
    Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts
    Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extracts
    Lane 7 : MDCK (Canine kidney cell line) cell extracts
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extracts
    Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extracts

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 57 kDa

  • Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for ATPB with ab230706 at a 1:50 dilution in immunohistochemical analysis.

  • All lanes : Anti-ATPB antibody [6C10] (ab230706) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY ATPB transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 57 kDa

References

ab230706 has not yet been referenced specifically in any publications.

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