Overview

  • Product name
    Anti-ATPB antibody [EPR11990]
    See all ATPB primary antibodies
  • Description
    Rabbit monoclonal [EPR11990] to ATPB
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ATPB aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: P06576

  • Positive control
    • HepG2; 293T; HeLa and HT-29 cell lysates; Human heart and liver tissues; HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/250.

Antigen retrieval is recommended.

ICC/IF 1/500.

For unpurified use at 1/50 - 1/100.

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
    • Sequence similarities
      Belongs to the ATPase alpha/beta chains family.
    • Cellular localization
      Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP 5B antibody
      • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
      • ATP synthase subunit beta mitochondrial antibody
      • ATP synthase subunit beta, mitochondrial antibody
      • atp5b antibody
      • ATPB antibody
      • ATPB_HUMAN antibody
      • ATPMB antibody
      • ATPSB antibody
      • Epididymis secretory protein Li 271 antibody
      • HEL-S-271 antibody
      • Mitochondrial ATP synthase beta subunit antibody
      • Mitochondrial ATP Synthase Subunit Beta antibody
      • Mitochondrial ATP synthetase beta subunit antibody
      see all

    Images

    • All lanes : Anti-ATPB antibody [EPR11990] (ab170947) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HT29 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 56 kDa

    • Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling ATPB with purified ab170947 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • ab170947 showing +ve staining in Human normal kidney tissue.

    • ab170947 showing +ve staining in Human normal uterus tissue.

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ATPB with ab170947 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ATPB with ab170947 at 1/100 dilution.

    • Immunofluorescent analysis of HeLa cells labeling ATPB using ab170947 at 1/50 dilution (green). DAPI nuclear staining (blue).

    • Anti-ATPB antibody [EPR11990] (ab170947) at 1/10 dilution + HepG2 cell lysate

      Secondary
      HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab170947 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MDA MB 231 cells)
    Permeabilization
    Yes - 1% Triton
    Specification
    MDA MB 231 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Fixative
    Formaldehyde

    Ms. Iglika Ivanova

    Verified customer

    Submitted Jan 14 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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