Recombinant
RabMAb

Anti-ATPB antibody [EPR11991] (ab170948)

Overview

  • Product name
    Anti-ATPB antibody [EPR11991]
    See all ATPB primary antibodies
  • Description
    Rabbit monoclonal [EPR11991] to ATPB
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATPB aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P06576

  • Positive control
    • HepG2, 293T, HeLa and JAR cell lysates; 293 cell lysate; 293 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
    • Sequence similarities
      Belongs to the ATPase alpha/beta chains family.
    • Cellular localization
      Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP 5B antibody
      • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
      • ATP synthase subunit beta mitochondrial antibody
      • ATP synthase subunit beta, mitochondrial antibody
      • atp5b antibody
      • ATPB antibody
      • ATPB_HUMAN antibody
      • ATPMB antibody
      • ATPSB antibody
      • Epididymis secretory protein Li 271 antibody
      • HEL-S-271 antibody
      • Mitochondrial ATP synthase beta subunit antibody
      • Mitochondrial ATP Synthase Subunit Beta antibody
      • Mitochondrial ATP synthetase beta subunit antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized 293 cells labeling ATP5B using ab170948 at 1/10 dilution (red) or a rabbit IgG negative (green).

    • All lanes : Anti-ATPB antibody [EPR11991] (ab170948) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 56 kDa

    • Anti-ATPB antibody [EPR11991] (ab170948) at 1/10 dilution + 293 cell lysate at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab170948 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Sample
    Human Cell (MDA MB 231 cells)
    Specification
    MDA MB 231 cells
    Permeabilization
    Yes - 1% Triton
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 05 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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