• Product name

    Anti-ATPB antibody [EPR11991]
    See all ATPB primary antibodies
  • Description

    Rabbit monoclonal [EPR11991] to ATPB
  • Host species

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATPB aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P06576

  • Positive control

    • HepG2, 293T, HeLa and JAR cell lysates; 293 cell lysate; 293 cells
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab170948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
    • Sequence similarities

      Belongs to the ATPase alpha/beta chains family.
    • Cellular localization

      Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATP 5B antibody
      • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
      • ATP synthase subunit beta mitochondrial antibody
      • ATP synthase subunit beta, mitochondrial antibody
      • atp5b antibody
      • ATPB antibody
      • ATPB_HUMAN antibody
      • ATPMB antibody
      • ATPSB antibody
      • Epididymis secretory protein Li 271 antibody
      • HEL-S-271 antibody
      • Mitochondrial ATP synthase beta subunit antibody
      • Mitochondrial ATP Synthase Subunit Beta antibody
      • Mitochondrial ATP synthetase beta subunit antibody
      see all


    • All lanes : Anti-ATPB antibody [EPR11991] (ab170948) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 56 kDa

    • Flow cytometric analysis of permeabilized 293 cells labeling ATP5B using ab170948 at 1/10 dilution (red) or a rabbit IgG negative (green).

    • Anti-ATPB antibody [EPR11991] (ab170948) at 1/10 dilution + 293 cell lysate at 10 µg

      HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.


    ab170948 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    This product is known to not work in this application or species.
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Human Cell (MDA MB 231 cells)
    MDA MB 231 cells
    Yes - 1% Triton

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    Verified customer

    Submitted Mar 05 2015

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