Anti-ATPBD3 antibody (ab136083)
Key features and details
- Rabbit polyclonal to ATPBD3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATPBD3 antibody
See all ATPBD3 primary antibodies -
Description
Rabbit polyclonal to ATPBD3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from the C terminal region of Human ATPBD3.
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Positive control
- 293 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 36 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 36 kDa. |
Target
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Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. -
Pathway
tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. -
Sequence similarities
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 90353 Human
- Omim: 612694 Human
- SwissProt: Q7Z7A3 Human
- Unigene: 148425 Human
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Alternative names
- ATP binding domain 3 antibody
- ATP-binding domain-containing protein 3 antibody
- Cancer associated protein antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab136083 has been referenced in 2 publications.
- Trzaska C et al. 2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations. Nat Commun 11:1509 (2020). PubMed: 32198346
- Xu S et al. Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer. Nat Commun 10:5492 (2019). PubMed: 31792210