Anti-ATPG antibody - C-terminal (ab190310)

Overview

  • Product name
    Anti-ATPG antibody - C-terminal
    See all ATPG primary antibodies
  • Description
    Goat polyclonal to ATPG - C-terminal
  • Host species
    Goat
  • Specificity
    ab190310 is expected to recognize both reported isoforms (NP_005165.1; NP_001001973.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human ATPG aa 272-286 (C terminal) (Cysteine residue). NP_001001973.1.
    Sequence:

    TLTFNRTRQAVITKE


    Database link: P36542

  • Positive control
    • Human Heart and in Rat Heart lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab190310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

Target

  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • Tissue specificity
    Isoform Heart is expressed specifically in the heart and skeletal muscle, which require rapid energy supply. Isoform Liver is expressed in the brain, liver and kidney. Isoform Heart and Isoform Liver are expressed in the skin, intestine, stomach and aorta.
  • Sequence similarities
    Belongs to the ATPase gamma chain family.
  • Cellular localization
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP synthase gamma chain, mitochondrial antibody
    • ATP synthase subunit gamma antibody
    • ATP synthase subunit gamma, mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, gamma subunit antibody
    • ATP5C 1 antibody
    • ATP5C antibody
    • ATP5C1 antibody
    • ATP5CL1 antibody
    • ATPG_HUMAN antibody
    • F ATPase gamma subunit antibody
    • F-ATPase gamma subunit antibody
    • mitochondrial antibody
    • Mitochondrial ATP synthase, gamma subunit 1 antibody
    see all

Images

  • Immunohistchemical analysis of paraffin embedded Human heart tissue, staining ATPG with ab190310 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Anti-ATPG antibody - C-terminal (ab190310) at 0.01 µg/ml + Rat Heart lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa

References

ab190310 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Drosophila melanogaster Cell (Spermatocyte in meiosis prophase I)
Permeabilization
Yes - 10 minutes, PBS Triton (0.4%)
Specification
Spermatocyte in meiosis prophase I
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 24 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up