• Product name

  • Description

    Rabbit polyclonal to ATR
  • Host species

  • Specificity

    We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human Ataxia and Rad3 related encoded within exon 5 (LocusLink ID 545).

  • Positive control

    • Whole cell lysate from HeLa and U2OS cells



Our Abpromise guarantee covers the use of ab10312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    WB: 1/15000 - 1/30000. Detects a band of approximately 300 kDa (predicted molecular weight: 317 kDa). Block membrane with 5% milk and incubate antibodies (primary and secondary) in milk.
    This antibody can often recognise extra bands which are fragments of ATR.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
    • Tissue specificity

      Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
    • Involvement in disease

      Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
    • Sequence similarities

      Belongs to the PI3/PI4-kinase family. ATM subfamily.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 2 HEAT repeats.
      Contains 1 PI3K/PI4K domain.
    • Post-translational

      Phosphorylated; autophosphorylates in vitro.
    • Cellular localization

      Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
    • Information by UniProt
    • Database links

    • Alternative names

      • Ataxia telangiectasia and Rad3 related antibody
      • Ataxia telangiectasia and Rad3-related protein antibody
      • ATR antibody
      • ATR_HUMAN antibody
      • FCTCS antibody
      • FRAP Related Protein 1 antibody
      • FRAP-related protein 1 antibody
      • FRP1 antibody
      • MEC1 antibody
      • MEC1 mitosis entry checkpoint 1 homolog antibody
      • Protein kinase ATR antibody
      • RAC3 antibody
      • Rad3 related protein antibody
      • SCKL antibody
      • SCKL1 antibody
      • Serine/threonine protein kinase ATR antibody
      • Serine/threonine-protein kinase ATR antibody
      see all


    • Detection of human ATR by Western Blot. Samples: Whole cell lysate (20 µg) from HeLa and U2OS cells separated on a 3 to 8% tris-acetate gel. Antibody: ab10312 used at 0.07 mg/ml. Detection: Chemiluminescence with 15 second exposure. Detection of human ATR by Western Blot. Samples: Whole cell lysate (20 µg) from HeLa and U2OS cells separated on a 3 to 8% tris-acetate gel. Antibody: ab10312 used at 0.07 mg/ml. Detection: Chemiluminescence with 15 second exposure.


    This product has been referenced in:

    • Bianco JN  et al. Overexpression of Claspin and Timeless protects cancer cells from replication stress in a checkpoint-independent manner. Nat Commun 10:910 (2019). Read more (PubMed: 30796221) »
    • Zhang P  et al. ATM-mediated stabilization of ZEB1 promotes DNA damage response and radioresistance through CHK1. Nat Cell Biol 16:864-75 (2014). Read more (PubMed: 25086746) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A


    Thank you for taking the time to fill out the technical questionaire and send it in to us. I apologise for the delay in getting back to you. However, I have more details of the western blot testing procedure for this antibody from the laboratory which I hope will help. This antibody can often recognise extra bands which are fragments of ATR, although this has yet to be tested on glioblastoma cells. If the small bands are more prevalent relative to the full-size ATR in this cell line, there may be a problem which we can resolve by optimisation. 1. The small bands may represent fragments of ATR. Ensure the lysate is fresh and correctly prepared. Ensure samples are adequately denatured and reduced. 2. The gels should be of lower percentage, 4 to 8%. 3. The primary antibody is at a high concentration which may lead to non specific binding. The data sheet suggests using a dilution of 1:15000 to 1:30000. There has been an Abreview submitted for this antibod in which the antibody was diluted 1:50000 with good results. 4. The amount of protein loaded is quite high. Try 20-30ug per lane. 5. You can try increasing the blocking incubation time to 2-3 hours. When the antibody was tested the laboratory, they used used 5% Non-fat Dry Milk in TBS with 0.05% Tween to block the membrane. The same was used as diluent for the primary and secondary antibody. I hope this is helpful information and that these suggestions will improve the results. Should you still obtain an unsatisfactory result after using these suggestions, then please do not hesitate to get back to me.

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    You recently enquired about our ATM and ATR antibodies and my colleague Karen has already sent you a reply regarding the antibodies ab2354 and ab4471. I have also investigated your enquiry regarding ab10312. The antibody should not react with ATM owing to complete divergence of amino acid sequence between ATM and ATR at the site to which the epitope maps. There is no indication that the antibody crossreacts with ATM. I hope this further information helps,

    Read More

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