Recombinant
RabMAb

Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)

Overview

  • Product name
    Anti-ATR (phospho S428) antibody [EPR2184]
    See all ATR primary antibodies
  • Description
    Rabbit monoclonal [EPR2184] to ATR (phospho S428)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATR aa 400-500 (phospho S428) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13535

  • Positive control
    • HeLa cell lysate treated with Okadaic Acid + Calyculin A, HEK-293 cell lysate; Human prostate and Human testis tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 300 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
    • Tissue specificity
      Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
    • Involvement in disease
      Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
    • Sequence similarities
      Belongs to the PI3/PI4-kinase family. ATM subfamily.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 2 HEAT repeats.
      Contains 1 PI3K/PI4K domain.
    • Post-translational
      modifications
      Phosphorylated; autophosphorylates in vitro.
    • Cellular localization
      Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ataxia telangiectasia and Rad3 related antibody
      • Ataxia telangiectasia and Rad3-related protein antibody
      • ATR antibody
      • ATR_HUMAN antibody
      • FCTCS antibody
      • FRAP Related Protein 1 antibody
      • FRAP-related protein 1 antibody
      • FRP1 antibody
      • MEC1 antibody
      • MEC1 mitosis entry checkpoint 1 homolog antibody
      • Protein kinase ATR antibody
      • RAC3 antibody
      • Rad3 related protein antibody
      • SCKL antibody
      • SCKL1 antibody
      • Serine/threonine protein kinase ATR antibody
      • Serine/threonine-protein kinase ATR antibody
      see all

    Images

    • All lanes : Anti-ATR (phospho S428) antibody [EPR2184] (ab178407) at 1/1000 dilution

      Lane 1 : Untreated HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates
      Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) treated with UV at 60J/m2 for 2 hours. whole cell lysates
      Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) treated with UV at 60J/m2 for 2 hours. whole cell lysates. Then the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 300 kDa
      Observed band size: 300 kDa


      Exposure time: 3 minutes


      Blocking/Diluting buffer 5% NFDM/TBST

    • Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling ATR (phospho S428) with ab178407 at 1/50.

    • All lanes : Anti-ATR (phospho S428) antibody [EPR2184] (ab178407) at 1/1000 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate treated with Okadaic Acid + Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 300 kDa

    • Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ATR (phospho S428) with ab178407 at 1/50.

    • Dot Blot analysis of Lane 1: ATR (pS428) phospho peptide and Lane 2: ATR non-phospho peptide labeling ATR (phospho S428) with ab178407 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 10 seconds.

    References

    This product has been referenced in:
    • Shi Q  et al. The identification of the ATR inhibitor VE-822 as a therapeutic strategy for enhancing cisplatin chemosensitivity in esophageal squamous cell carcinoma. Cancer Lett 432:56-68 (2018). WB . Read more (PubMed: 29890208) »
    • Zhang C  et al. Radioresistance of chordoma cells is associated with the ATM/ATR pathway, in which RAD51 serves as an important downstream effector. Exp Ther Med 14:2171-2179 (2017). Read more (PubMed: 28962138) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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