IHC-P, WBmore details Unsuitable for:
Flow Cyt,ICC/IF or IP
Human Does not react with:
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATR aa 400-500 (phospho S428) (Cysteine residue). The exact sequence is proprietary. Database link: Q13535
HeLa cell lysate treated with Okadaic Acid + Calyculin A, HEK-293 cell lysate; Human prostate and Human testis tissues.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
Involvement in disease
Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
Ataxia telangiectasia and Rad3-related protein antibody
FRAP Related Protein 1 antibody
FRAP-related protein 1 antibody
MEC1 mitosis entry checkpoint 1 homolog antibody
Protein kinase ATR antibody
Rad3 related protein antibody
Serine/threonine protein kinase ATR antibody
Serine/threonine-protein kinase ATR antibody
Western blot - Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)
All lanes : Anti-ATR (phospho S428) antibody [EPR2184] (ab178407) at 1/1000 dilution
Lane 1 : Untreated HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) treated with UV at 60J/m2 for 2 hours. whole cell lysates Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) treated with UV at 60J/m2 for 2 hours. whole cell lysates. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 300 kDa Observed band size: 300 kDa
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ATR (phospho S428) with ab178407 at 1/50.
Dot Blot - Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)
Dot Blot analysis of Lane 1: ATR (pS428) phospho peptide and Lane 2: ATR non-phospho peptide labeling ATR (phospho S428) with ab178407 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 10 seconds.
Shi Q et al. The identification of the ATR inhibitor VE-822 as a therapeutic strategy for enhancing cisplatin chemosensitivity in esophageal squamous cell carcinoma. Cancer Lett432:56-68 (2018).
Read more (PubMed: 29890208) »
Zhang C et al. Radioresistance of chordoma cells is associated with the ATM/ATR pathway, in which RAD51 serves as an important downstream effector. Exp Ther Med14:2171-2179 (2017).
Read more (PubMed: 28962138) »