Recombinant
RabMAb

Recombinant Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

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Overview

  • Product name

    Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free
    See all ATR primary antibodies

  • Description

    Rabbit monoclonal [EPR2184] to ATR (phospho S428) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATR aa 400-500 (phospho S428) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13535

  • Positive control

    • HeLa cell lysate treated with Okadaic Acid + Calyculin A, HEK-293 cell lysate; Human prostate and Human testis tissues.

  • General notes

    Ab230831 is the carrier-free version of ab178407. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab230831 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab230831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 300 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot blot Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

    • Tissue specificity

      Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.

    • Involvement in disease

      Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.

    • Sequence similarities

      Belongs to the PI3/PI4-kinase family. ATM subfamily.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 2 HEAT repeats.
      Contains 1 PI3K/PI4K domain.

    • Post-translational
      modifications

      Phosphorylated; autophosphorylates in vitro.

    • Cellular localization

      Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
    • Information by UniProt

    • Database links

      • Alternative names

        • Ataxia telangiectasia and Rad3 related antibody
        • Ataxia telangiectasia and Rad3-related protein antibody
        • ATR antibody
        • ATR_HUMAN antibody
        • FCTCS antibody
        • FRAP Related Protein 1 antibody
        • FRAP-related protein 1 antibody
        • FRP1 antibody
        • MEC1 antibody
        • MEC1 mitosis entry checkpoint 1 homolog antibody
        • Protein kinase ATR antibody
        • RAC3 antibody
        • Rad3 related protein antibody
        • SCKL antibody
        • SCKL1 antibody
        • Serine/threonine protein kinase ATR antibody
        • Serine/threonine-protein kinase ATR antibody
        see all

      Images

      • Dot Blot - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
        Dot Blot - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

        Dot Blot analysis of Lane 1: ATR (pS428) phospho peptide and Lane 2: ATR non-phospho peptide labeling ATR (phospho S428) with ab178407 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 10 seconds.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178407).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

        This IHC data was generated using the same phospho anti-ATR antibody clone, EPR2184, in a different buffer formulation (cat# ab178407).

        Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling ATR (phospho S428) with ab178407 at 1/50.

        Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

        This IHC data was generated using the same phospho anti-ATR antibody clone, EPR2184, in a different buffer formulation (cat# ab178407).

        Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ATR (phospho S428) with ab178407 at 1/50.

        Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Di Benedetto A  et al. Analysis of the ATR-Chk1 and ATM-Chk2 pathways in male breast cancer revealed the prognostic significance of ATR expression. Sci Rep 7:8078 (2017). IHC . Read more (PubMed: 28808232) »
      See 1 Publication for this product

      Publishing research using ab230831? Please let us know so that we can cite the reference in this datasheet.

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