RecombinantRabMAb

Recombinant Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

Submit a review Submit a question References (1)
Dot Blot - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
  • Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2184] to ATR (phospho S428) - BSA and Azide free
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free
    See all ATR primary antibodies

  • Description

    Rabbit monoclonal [EPR2184] to ATR (phospho S428) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATR aa 400-500 (phospho S428) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13535

  • Positive control

    • HeLa cell lysate treated with Okadaic Acid + Calyculin A, HEK-293 cell lysate; Human prostate and Human testis tissues.

  • General notes

    Ab230831 is the carrier-free version of ab178407. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab230831 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab230831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 300 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot blot Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

    • Tissue specificity

      Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.

    • Involvement in disease

      Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.

    • Sequence similarities

      Belongs to the PI3/PI4-kinase family. ATM subfamily.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 2 HEAT repeats.
      Contains 1 PI3K/PI4K domain.

    • Post-translational
      modifications

      Phosphorylated; autophosphorylates in vitro.

    • Cellular localization

      Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
    • Information by UniProt

    • Database links

      • Alternative names

        • Ataxia telangiectasia and Rad3 related antibody
        • Ataxia telangiectasia and Rad3-related protein antibody
        • ATR antibody
        • ATR_HUMAN antibody
        • FCTCS antibody
        • FRAP Related Protein 1 antibody
        • FRAP-related protein 1 antibody
        • FRP1 antibody
        • MEC1 antibody
        • MEC1 mitosis entry checkpoint 1 homolog antibody
        • Protein kinase ATR antibody
        • RAC3 antibody
        • Rad3 related protein antibody
        • SCKL antibody
        • SCKL1 antibody
        • Serine/threonine protein kinase ATR antibody
        • Serine/threonine-protein kinase ATR antibody
        see all

      Images

      • Dot Blot - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
        Dot Blot - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

        Dot Blot analysis of Lane 1: ATR (pS428) phospho peptide and Lane 2: ATR non-phospho peptide labeling ATR (phospho S428) with ab178407 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 10 seconds.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178407).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

        This IHC data was generated using the same phospho anti-ATR antibody clone, EPR2184, in a different buffer formulation (cat# ab178407).

        Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling ATR (phospho S428) with ab178407 at 1/50.

        Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

        This IHC data was generated using the same phospho anti-ATR antibody clone, EPR2184, in a different buffer formulation (cat# ab178407).

        Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ATR (phospho S428) with ab178407 at 1/50.

        Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      • Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
        Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (1)

      Publishing research using ab230831? Please let us know so that we can cite the reference in this datasheet.

      ab230831 has been referenced in 1 publication.

      • Di Benedetto A  et al. Analysis of the ATR-Chk1 and ATM-Chk2 pathways in male breast cancer revealed the prognostic significance of ATR expression. Sci Rep 7:8078 (2017). IHC . PubMed: 28808232

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab230831.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com