Overview

  • Product name

    Anti-ATR (phospho T1989) antibody
    See all ATR primary antibodies
  • Description

    Rabbit polyclonal to ATR (phospho T1989)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ATR (C terminal) (phospho T1989). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: Q13535

  • Positive control

    • WB: Raji nuclear and whole cell extracts; HCT 116 whole cell extracts treated with/without hydroxyurea. IP: HCT 116 whole cell extract. IHC-P: Human lung, ovarian carcinoma, breast carcinoma, colon cancer and lung cancer tissues; Mouse heart and liver tissues. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 301 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
  • Tissue specificity

    Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
  • Involvement in disease

    Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family. ATM subfamily.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 2 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • Post-translational
    modifications

    Phosphorylated; autophosphorylates in vitro.
  • Cellular localization

    Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ataxia telangiectasia and Rad3 related antibody
    • Ataxia telangiectasia and Rad3-related protein antibody
    • ATR antibody
    • ATR_HUMAN antibody
    • FCTCS antibody
    • FRAP Related Protein 1 antibody
    • FRAP-related protein 1 antibody
    • FRP1 antibody
    • MEC1 antibody
    • MEC1 mitosis entry checkpoint 1 homolog antibody
    • Protein kinase ATR antibody
    • RAC3 antibody
    • Rad3 related protein antibody
    • SCKL antibody
    • SCKL1 antibody
    • Serine/threonine protein kinase ATR antibody
    • Serine/threonine-protein kinase ATR antibody
    see all

Images

  • All lanes : Anti-ATR (phospho T1989) antibody (ab227851) at 1/500 dilution

    Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 301 kDa



    5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ATR (phospho T1989) (green) using ab227851 at 1/500 dilution in ICC/IF.

    Counterstain: phalloidin cytoskeleton marker (red).

  • Paraffin-embedded human lung tissue stained for ATR (phospho T1989) using ab227851 at 1/250 dilution in immunohistochemical analysis.

  • ATR (phospho T1989) was immunoprecipitated from from HCT 116 (human colorectal carcinoma cell line) whole cell extract treated with hydroxyurea for 6 hours using 5 μg ab227851. Western blot analysis was performed using ab227851.

    Lane 1: HCT 116 whole cell extract treated with hydroxyurea for 6 hours.

    Lane 2: Control IgG in HCT 116 whole cell extract treated with hydroxyurea for 6 hours.

    Lane 3: ab227851 in HCT 116 whole cell extract treated with hydroxyurea for 6 hours.

     

  • Paraffin-embedded mouse liver tissue stained for ATR (phospho T1989) using ab227851 at 1/250 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse heart tissue stained for ATR (phospho T1989) using ab227851 at 1/250 dilution in immunohistochemical analysis.

  • Paraffin-embedded human lung cancer tissue stained for ATR (phospho T1989) using ab227851 at 1/250 dilution in immunohistochemical analysis.

  • Paraffin-embedded human ovarian carcinoma tissue stained for ATR (phospho T1989) using ab227851 at 1/250 dilution in immunohistochemical analysis.

  • Paraffin-embedded human breast carcinoma tissue stained for ATR (phospho T1989) using ab227851 at 1/250 dilution in immunohistochemical analysis.

  • Paraffin-embedded human colon cancer tissue stained for ATR (phospho T1989) using ab227851 at 1/250 dilution in immunohistochemical analysis.

  • All lanes : Anti-ATR (phospho T1989) antibody (ab227851) at 1/1000 dilution

    Lane 1 : Untreated HCT 116 (human colorectal carcinoma cell line) whole cell extract
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) treated with 1 mM hydroxyurea for 6 hours, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 301 kDa



    5% SDS-PAGE gel

References

This product has been referenced in:

  • Wang WY  et al. HMGA2 gene silencing reduces epithelial-mesenchymal transition and lymph node metastasis in cervical cancer through inhibiting the ATR/Chk1 signaling pathway. Am J Transl Res 10:3036-3052 (2018). Read more (PubMed: 30416649) »
See 1 Publication for this product

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