• Product name

    Anti-ATR (phospho T1989) antibody [EPR21991]
    See all ATR primary antibodies
  • Description

    Rabbit monoclonal [EPR21991] to ATR (phospho T1989)
  • Host species

  • Tested applications

    Suitable for: WB, Dot blotmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ATR aa 1950-2050 (phospho T1989). The exact sequence is proprietary.
    Database link: Q13535

  • Positive control

    • WB: Untreated HEK-293 whole cell lysate; HEK-293 cell lysate treated with 2mM hydroxyurea for 16 hours.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab223258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 301 kDa (predicted molecular weight: 301 kDa).
Dot blot 1/1000.


  • Function

    Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
  • Tissue specificity

    Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
  • Involvement in disease

    Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family. ATM subfamily.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 2 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • Post-translational

    Phosphorylated; autophosphorylates in vitro.
  • Cellular localization

    Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ataxia telangiectasia and Rad3 related antibody
    • Ataxia telangiectasia and Rad3-related protein antibody
    • ATR antibody
    • ATR_HUMAN antibody
    • FCTCS antibody
    • FRAP Related Protein 1 antibody
    • FRAP-related protein 1 antibody
    • FRP1 antibody
    • MEC1 antibody
    • MEC1 mitosis entry checkpoint 1 homolog antibody
    • Protein kinase ATR antibody
    • RAC3 antibody
    • Rad3 related protein antibody
    • SCKL antibody
    • SCKL1 antibody
    • Serine/threonine protein kinase ATR antibody
    • Serine/threonine-protein kinase ATR antibody
    see all


  • All lanes : Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258) at 1/1000 dilution

    Lane 1 : Untreated HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : HEK-293 treated with 2mM hydroxyurea for 16 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 301 kDa
    Observed band size: 301 kDa

    Exposure time: 3 minutes

    Blocking/Dilution buffer: 2% BSA/TBST

    ATR Thr1989 phosphorylation is increased after DNA damage (PMID: 21777809).

  • Dot blot analysis of ATR (phospho T1989) labeled with ab223258 at 1/1000 dilution.

    Lane 1: ATR (phospho T1989) peptide (aa1983-1992).
    Lane 2: ATR (phospho T1989) peptide (aa1986-1995).
    Lane 3: ATR non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.


ab223258 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Western blot
Human Cell lysate - whole cell (HAP1)
Gel Running Conditions
Reduced Denaturing (6)
Loading amount
20 µg
Hydroxyurea 20hr
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Sep 04 2019

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