Recombinant Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)
![Western blot - Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)](https://www.abcam.com/ps/products/223/ab223258/Images/ab223258-486398-anti-atr-phospho-t1989-antibody-epr21991-western-blot-hela-cell-lysate.jpg "Western blot - Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21991] to ATR (phospho T1989)
- Suitable for: WB, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ATR (phospho T1989) antibody [EPR21991]
See all ATR primary antibodies -
Description
Rabbit monoclonal [EPR21991] to ATR (phospho T1989) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blotmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Untreated HeLa whole cell lysate; HeLa cell lysate treated with 4mM hydroxyurea for 20 hour.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21991 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 301 kDa (predicted molecular weight: 301 kDa).
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| Dot blot |
1/1000.
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 301 kDa (predicted molecular weight: 301 kDa). |
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Dot blot
1/1000. |
Target
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Function
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. -
Tissue specificity
Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary. -
Involvement in disease
Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. -
Sequence similarities
Belongs to the PI3/PI4-kinase family. ATM subfamily.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain. -
Post-translational
modificationsPhosphorylated; autophosphorylates in vitro. -
Cellular localization
Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling. - Information by UniProt
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Database links
- Entrez Gene: 545 Human
- Omim: 601215 Human
- SwissProt: Q13535 Human
- Unigene: 271791 Human
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Alternative names
- Ataxia telangiectasia and Rad3 related antibody
- Ataxia telangiectasia and Rad3-related protein antibody
- ATR antibody
see all
Images
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Western blot - Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)All lanes : Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with 4mM hydroxyurea for 20 hours whole cell lysate
Lane 3 : HeLa treated with 4mM hydroxyurea for 20 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 4 : HeLa treated with 4mM hydroxyurea for 20 hours whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 301 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?
Exposure time: 100 secondsBlocking and dilution buffer: 5% NFDM/TBST.
We are unsure of the nature of the 110kDa band.
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![Dot Blot - Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)](https://www.abcam.com/ps/products/223/ab223258/Images/ab223258-314781-anti-atr-phospho-t1989-antibody-epr21991-dot-blot.jpg)
Dot Blot - Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)Dot blot analysis of ATR (phospho T1989) labeled with ab223258 at 1/1000 dilution.
Lane 1: ATR (phospho T1989) peptide (aa1983-1992).
Lane 2: ATR (phospho T1989) peptide (aa1986-1995).
Lane 3: ATR non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)](https://www.abcam.com/ps/products/223/ab223258/Images/ab223258-3-benefits-of-recombinant-antibodies.png)
Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab223258 has not yet been referenced specifically in any publications.
