Application
Western blot
Sample
Human Cell lysate - whole cell (HAP1)
Gel Running Conditions
Reduced Denaturing (6)
Loading amount
20 µg
Treatment
Hydroxyurea 20hr
Specification
HAP1
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk / PBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Swine anti Rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: +250 kDa
Abcam user community
Verified customer
Submitted Sep 04 2019