Product nameAnti-AtRAC3 antibody
DescriptionRabbit polyclonal to AtRAC3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Arabidopsis thaliana
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab50678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
RelevancePlants contain a unique Rho GTPase subfamily designated as RAC or Rop (Rho from plants), subdivided into two major subgroups according to whether they are prenylated or palmitoylated. RAC/Rop proteins regulate a wide array of cellular processes. They induce reorganization of actin, and have been implicated in changes in polar growth patterns, inducing Ca2+ influx at the tip in germinating pollen tubes, regulating various hormone-mediated plant signalling and developmental processes, the production of reactive oxygen species and modulating the pathogen-induced hypersensitive response (HR). In Arabidopsis, 11 different RAC/Rop GTPases have been identified so far, but the function of the individual RACs is not yet fully understood.
Cellular localizationCytoplasmic and Cell Membrane; peripheral membrane protein. Note: Associated with the membrane when activated.
- ARAC3 antibody
- ATROP6 antibody
- GTPase protein ROP6 antibody
All lanes : Anti-AtRAC3 antibody (ab50678) at 1/2000 dilution
Lane 1 : E. coli extract expressing AtRAC3 (10µg)
Lane 2 : E. coli extract expressing AtRAC3 (5µg)
Lane 3 : E. coli extract expressing AtRAC3 (2.5µg)
Lane 4 : E. coli extract expressing AtRAC3 (1.2µg)
Lane 5 : E. coli extract expressing AtRAC3 (0.6µg)
Lane 6 : E. coli extract expressing AtRAC3 (5mg) with immunizing peptide at 10 µg/ml
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate
Developed using the ECL technique.
Predicted band size: 22 kDa
Observed band size: 22 kDa
ab50678 has not yet been referenced specifically in any publications.