The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Plants contain a unique Rho GTPase subfamily designated as RAC or Rop (Rho from plants), subdivided into two major subgroups according to whether they are prenylated or palmitoylated. RAC/Rop proteins regulate a wide array of cellular processes. They induce reorganization of actin, and have been implicated in changes in polar growth patterns, inducing Ca2+ influx at the tip in germinating pollen tubes, regulating various hormone-mediated plant signalling and developmental processes, the production of reactive oxygen species and modulating the pathogen-induced hypersensitive response (HR). In Arabidopsis, 11 different RAC/Rop GTPases have been identified so far, but the function of the individual RACs is not yet fully understood.
Cytoplasmic and Cell Membrane; peripheral membrane protein. Note: Associated with the membrane when activated.
All lanes : Anti-AtRAC3 antibody (ab50678) at 1/2000 dilution
Lane 1 : E. coli extract expressing AtRAC3 (10µg) Lane 2 : E. coli extract expressing AtRAC3 (5µg) Lane 3 : E. coli extract expressing AtRAC3 (2.5µg) Lane 4 : E. coli extract expressing AtRAC3 (1.2µg) Lane 5 : E. coli extract expressing AtRAC3 (0.6µg) Lane 6 : E. coli extract expressing AtRAC3 (5mg) with immunizing peptide at 10 µg/ml
Secondary All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate
Developed using the ECL technique.
Predicted band size: 22 kDa Observed band size: 22 kDa