Anti-ATRAP antibody (ab85175)
Key features and details
- Rabbit polyclonal to ATRAP
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATRAP antibody
See all ATRAP primary antibodies -
Description
Rabbit polyclonal to ATRAP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ATRAP (C terminal).
Database link: NM_020350 -
Positive control
- WB: HeLa, HepG2, SK0V3, MCF-7 and MDA-MD-231 cell lysates. IHC-P: Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute in 200ul sterile H2O. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200.
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WB |
1/200 - 1/1000. Predicted molecular weight: 17 kDa.
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Notes |
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IHC-P
1/50 - 1/200. |
WB
1/200 - 1/1000. Predicted molecular weight: 17 kDa. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 57085 Human
- Omim: 608729 Human
- SwissProt: Q6RW13 Human
- Unigene: 464438 Human
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Alternative names
- Agtrap antibody
- Angiotensin II receptor associated protein antibody
- Angiotensin II type I receptor associated protein antibody
see all
Images
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All lanes : Anti-ATRAP antibody (ab85175) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : SK0V3 cell lysate
Lane 4 : MCF-7 cell lysate
Lane 5 : MDA-MD-231 cell lysate
Predicted band size: 17 kDa -
ab85175, at 1/100 dilution, staining ATRAP in formalin-fixed, paraffin-embedded human kidney by Immunohistochemistry. Note cytoplasmic staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab85175 has been referenced in 2 publications.
- Wang D et al. USF1-ATRAP-PBX3 Axis Promote Breast Cancer Glycolysis and Malignant Phenotype by Activating AKT/mTOR Signaling. Int J Biol Sci 18:2452-2471 (2022). PubMed: 35414770
- Yamaji T et al. Angiotensin II type 1 receptor-associated protein deficiency attenuates sirtuin1 expression in an immortalised human renal proximal tubule cell line. Sci Rep 9:16550 (2019). PubMed: 31719572