Overview

  • Product name

  • Description

    Mouse polyclonal to ATRAP
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human ATRAP aa 1-152.
    Database link: NP_001035284.1

  • Positive control

    • HeLa nuclear extract and ATRAP transfected 293T cell lysate.
  • General notes

     This product was previously labelled as AGTRAP

     

Properties

Applications

Our Abpromise guarantee covers the use of ab89247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Information by UniProt
  • Database links

  • Alternative names

    • Agtrap antibody
    • Angiotensin II receptor associated protein antibody
    • Angiotensin II type I receptor associated protein antibody
    • AT1 receptor-associated protein antibody
    • ATI receptor associated protein antibody
    • ATRAP antibody
    • ATRAP_HUMAN antibody
    • MGC29646 antibody
    • OTTHUMP00000002269 antibody
    • OTTHUMP00000002270 antibody
    • OTTHUMP00000002271 antibody
    • Type 1 angiotensin II receptor associated protein antibody
    • Type-1 angiotensin II receptor-associated protein antibody
    see all

Images

  • Anti-ATRAP antibody (ab89247) at 1 µg/ml + HeLa nuclear extract at 50 µg

    Predicted band size: 17 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-ATRAP antibody (ab89247) at 1 µg/ml

    Lane 1 : ATRAP transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate.

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

References

ab89247 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
kidney
Blocking step
Blocking reagens Fa.Zytomed as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 01 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (eye)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
eye
Blocking step
Fa.Zytomed as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 01 2013

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