Anti-ATRIP antibody (ab245632)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ATRIP antibody
See all ATRIP primary antibodies -
Description
Rabbit polyclonal to ATRIP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human ATRIP aa 100-150. The exact sequence is proprietary. NP_569055.1
Database link: Q8WXE1 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245632 was affinity purified using an epitope specific to ATRIP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab245632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 86 kDa. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| IP | Use at 1-4 µg/mg of lysate. |
Target
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Function
Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the ATRIP family. -
Domain
The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage. -
Post-translational
modificationsPhosphorylated by ATR. -
Cellular localization
Nucleus. Redistributes to discrete nuclear foci upon DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 84126 Human
- Omim: 606605 Human
- SwissProt: Q8WXE1 Human
- Unigene: 694840 Human
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Alternative names
- AGS1 antibody
- ATIP antibody
- ATM and Rad3 related interacting protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRIP antibody (ab245632)Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for ATRIP using ab245632 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
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Western blot - Anti-ATRIP antibody (ab245632)All lanes : Anti-ATRIP antibody (ab245632) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-ATRIP antibody (ab245632)ATRIP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245632 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245632 at 1 µg/ml.
Lane 1: ab245632 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Datasheets and documents
References
ab245632 has not yet been referenced specifically in any publications.
