Key features and details
- Rabbit polyclonal to ATRIP
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATRIP antibody
See all ATRIP primary antibodies
DescriptionRabbit polyclonal to ATRIP
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ATRIP aa 100-150. The exact sequence is proprietary. NP_569055.1
Database link: Q8WXE1
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245632 was affinity purified using an epitope specific to ATRIP immobilized on solid support.
Our Abpromise guarantee covers the use of ab245632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 86 kDa.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|IP||Use at 1-4 µg/mg of lysate.|
FunctionRequired for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
Sequence similaritiesBelongs to the ATRIP family.
DomainThe EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
modificationsPhosphorylated by ATR.
Cellular localizationNucleus. Redistributes to discrete nuclear foci upon DNA damage.
- Information by UniProt
- AGS1 antibody
- ATIP antibody
- ATM and Rad3 related interacting protein antibody
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for ATRIP using ab245632 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
All lanes : Anti-ATRIP antibody (ab245632) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 30 seconds
ATRIP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245632 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245632 at 1 µg/ml.
Lane 1: ab245632 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245632 has not yet been referenced specifically in any publications.