Anti-ATRIP antibody (ab245632)
Key features and details
- Rabbit polyclonal to ATRIP
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATRIP antibody
See all ATRIP primary antibodies -
Description
Rabbit polyclonal to ATRIP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human ATRIP aa 100-150. The exact sequence is proprietary. NP_569055.1
Database link: Q8WXE1 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245632 was affinity purified using an epitope specific to ATRIP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab245632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 86 kDa. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| IP | Use at 1-4 µg/mg of lysate. |
Target
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Function
Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the ATRIP family. -
Domain
The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage. -
Post-translational
modificationsPhosphorylated by ATR. -
Cellular localization
Nucleus. Redistributes to discrete nuclear foci upon DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 84126 Human
- Omim: 606605 Human
- SwissProt: Q8WXE1 Human
- Unigene: 694840 Human
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Alternative names
- AGS1 antibody
- ATIP antibody
- ATM and Rad3 related interacting protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRIP antibody (ab245632)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for ATRIP using ab245632 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
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Western blot - Anti-ATRIP antibody (ab245632)All lanes : Anti-ATRIP antibody (ab245632) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-ATRIP antibody (ab245632)ATRIP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245632 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245632 at 1 µg/ml.
Lane 1: ab245632 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab245632 has not yet been referenced specifically in any publications.
