Overview

  • Product name
    Anti-ATRIP antibody [EPR8072(2)]
    See all ATRIP primary antibodies
  • Description
    Rabbit monoclonal [EPR8072(2)] to ATRIP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ATRIP aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8WXE1

  • Positive control
    • PC-3, Hela, MCF-7 cell lysates; MCF-7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR8072(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/5000. Predicted molecular weight: 86 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the ATRIP family.
    • Domain
      The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
    • Post-translational
      modifications
      Phosphorylated by ATR.
    • Cellular localization
      Nucleus. Redistributes to discrete nuclear foci upon DNA damage.
    • Information by UniProt
    • Database links
    • Alternative names
      • AGS1 antibody
      • ATIP antibody
      • ATM and Rad3 related interacting protein antibody
      • ATM and Rad3-related-interacting protein antibody
      • ATR interacting protein antibody
      • ATR-interacting protein antibody
      • Atrip antibody
      • ATRIP_HUMAN antibody
      • DKFZp762J2115 antibody
      • FLJ12343 antibody
      • MGC20625 antibody
      • MGC21482 antibody
      • MGC26740 antibody
      see all

    Images

    • All lanes : Anti-ATRIP antibody [EPR8072(2)] (ab175221) at 1/1000 dilution

      Lane 1 : PC-3 cell lysate
      Lane 2 : Hela cell lysate
      Lane 3 : MCF-7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 86 kDa

    • Immunofluorescent staining of MCF-7 cells labeling ATRIP with ab175221 at 1/50 dilution.

    References

    This product has been referenced in:
    • Hopkins J  et al. Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes. PLoS Genet 10:e1004413 (2014). ICC/IF ; Mouse . Read more (PubMed: 24992337) »

    See 1 Publication for this product

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    There are currently no Customer reviews or Questions for ab175221.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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