Product nameAnti-ATRIP antibody [EPR8072(2)]
See all ATRIP primary antibodies
DescriptionRabbit monoclonal [EPR8072(2)] to ATRIP
Tested applicationsSuitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ATRIP aa 200-300 (Cysteine residue). The exact sequence is proprietary.
Database link: Q8WXE1
- PC-3, Hela, MCF-7 cell lysates; MCF-7 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 86 kDa.|
FunctionRequired for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
Sequence similaritiesBelongs to the ATRIP family.
DomainThe EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
modificationsPhosphorylated by ATR.
Cellular localizationNucleus. Redistributes to discrete nuclear foci upon DNA damage.
- Information by UniProt
- AGS1 antibody
- ATIP antibody
- ATM and Rad3 related interacting protein antibody
All lanes : Anti-ATRIP antibody [EPR8072(2)] (ab175221) at 1/1000 dilution
Lane 1 : PC-3 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 86 kDa
Immunofluorescent staining of MCF-7 cells labeling ATRIP with ab175221 at 1/50 dilution.
This product has been referenced in:
- Hopkins J et al. Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes. PLoS Genet 10:e1004413 (2014). ICC/IF ; Mouse . Read more (PubMed: 24992337) »