Key features and details
- Rabbit polyclonal to Atrophin 1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Atrophin 1 antibody
See all Atrophin 1 primary antibodies
DescriptionRabbit polyclonal to Atrophin 1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- This antibody gave a positive signal in Human Testis Tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab32604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 125 kDa).|
RelevanceAtrophin 1 (ATN1) is essential for the development of multiple tissues. Defects in ATN1 are the cause of dentatorubral-pallidoluysian atrophy (DRPLA).
Cellular localizationCytoplasm. Nuclear
- ATN1 antibody
- Atrophin1 antibody
- B37 antibody
IHC image of ab32604 staining in Human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32604, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-Atrophin 1 antibody (ab32604) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 125 kDa
Observed band size: 125 kDa
Additional bands at: 100 kDa, 55 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
ab32604 has not yet been referenced specifically in any publications.