• Product name

  • Description

    Rabbit polyclonal to ATRX
  • Host species

  • Tested applications

    Suitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human ATRX aa 2211-2413.
    Database link: P46100

  • Positive control

    • 293T, HepG2, Raji, NIH-3T3, HeLa A431, H1299 and HeLaS3 cell lines. A549 xenograft.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab97508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 283 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.


  • Function

    Involved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells which probably implies recruitment of CBX5 to telomers. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting; according to a report, it is not sufficient to decrease chromatin condensation at telomers nor to increase expression of telomeric RNA in fibroblasts (PubMed:24500201). May be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. Acts as negative regulator of chromatin incorporation of transcriptionally repressive histone H2AFY, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. May be involved in brain development and facial morphogenesis. Binds to zinc-finger coding genes with atypical chromatin signatures and regulates its H3K9me3 levels. Forms a complex with ZNF274, TRIM28 and SETDB1 to facilitate the deposition and maintenance of H3K9me3 at the 3' exons of zinc-finger genes (PubMed:27029610).
  • Tissue specificity

  • Involvement in disease

    Alpha-thalassemia mental retardation syndrome, X-linked
    Mental retardation, X-linked, syndromic, with hypotonic facies 1
    Alpha-thalassemia myelodysplasia syndrome
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PHD-type zinc finger.
  • Domain

    The ADD domain predominantly interacts with histone H3 trimethylated at 'Lys-10'(H3K9me3) (and to a lesser extent H3 mono-or dimethylated at 'Lys-10') and simultanously to histone H3 unmethylated at 'Lys-5' (H3K4me0). The interaction with H3K9me3 is disrupted by the presence of H3K4me3 suggesting a readout of the combined histone H3 methylation state.
    Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
  • Post-translational

    Phosphorylated at serine residues during mitose. Phosphorylation may promote the release from the nuclear matrix and progression to mitosis.
  • Cellular localization

    Nucleus. Chromosome, telomere. Nucleus, PML body. Associated with pericentromeric heterochromatin during interphase and mitosis, probably by interacting with CBX5/HP1 alpha. Colocalizes with histone H3.3, DAXX, HIRA and ASF1A at PML-nuclear bodies. Colocalizes with cohesin (SMC1 and SMC3) and MECP2 at the maternal H19 ICR (By similarity).
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha thalassemia/mental retardation syndrome X linked homolog antibody
    • ATP dependent helicase ATRX antibody
    • ATP-dependent helicase ATRX antibody
    • ATR2 antibody
    • Atrx antibody
    • ATRX_HUMAN antibody
    • DNA dependent ATPase and helicase antibody
    • Helicase 2, X linked antibody
    • MGC2094 antibody
    • MRXHF1 antibody
    • RAD54 antibody
    • RAD54L antibody
    • SFM1 antibody
    • SHS antibody
    • Transcriptional regulator ATRX antibody
    • X linked helicase II antibody
    • X linked nuclear protein antibody
    • X-linked helicase II antibody
    • X-linked nuclear protein antibody
    • XH2 antibody
    • XNP antibody
    • Znf HX antibody
    • Znf-HX antibody
    see all


  • Western blot of (lane1) PC12 (brain)whole cell extracts, (lane 2) Rat 2 (embryo) whole cell extracts, at 30µg per lane, labeling ATRX with ab97508 at 1:1000.  A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)  analysis of human breast carcinoma labeling ATRX with ab97508 at 1/500 dilution. Anti-ATRX antibody (ab97508) detects ATRX protein at nucleus on human breast carcinoma tissue sections.

  • All lanes : Anti-ATRX antibody (ab97508) at 1/500 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 283 kDa

    5% SDS-PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of endometrial carcinoma labeling ATRX with ab97508 at 1/500 dilution. Anti-ATRX antibody (ab97508) detects ATRX protein at nucleus on human endometrial carcinoma tissue sections.

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma,using ab97508 at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence analysis of mouse brain labeling ATRX with ab97508 at 1/100 dilution. Left- DAPI (blue), middle - ATRX protein staining (red), right - merge.

  • ab97508 at 5µg immunoprecipitating ATRX in 293T whole cell extracts.

    Lane 1 (input): 293T whole cell extracts.
    Lane 2 (-): Rabbit IgG instead of ab97508 in 293T whole cell extracts.
    Lane 3 (+): Anti-ATRX antibody (ab97508) + 293T whole cell extracts.

  • Anti-ATRX antibody (ab97508) at 1/1000 dilution + NIH3T3 whole cell extracts at 30 µg

    Predicted band size: 283 kDa

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97508 at 1/200 dilution. Lower image is merged with DNA probe.
  • Anti-ATRX antibody (ab97508) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size: 283 kDa

    5% SDS-PAGE


This product has been referenced in:

  • Merkl PE & Knipe DM Role for a Filamentous Nuclear Assembly of IFI16, DNA, and Host Factors in Restriction of Herpesviral Infection. MBio 10:N/A (2019). Read more (PubMed: 30670617) »
  • Cavalcante SG  et al. ATRX-DAXX Complex Expression Levels and Telomere Length in Normal Young and Elder Autopsy Human Brains. DNA Cell Biol 38:955-961 (2019). Read more (PubMed: 31361513) »
See all 13 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Human Cell lysate - whole cell (Neuroblastoma)
Negative control
For negative control we use Input DNA from the same sample.
Detection step
Cross-linking (X-ChIP)
Duration of cross-linking step: 45 minute(s) and 0 second(s)
Specification of the cross-linking agent: Crosslinked with DSG for 45 mi
Positive control
We have done ChIP followed by Illumina sequencing and obtained very specific peaks with high signal to noise ratio. ChIP is done with about 40M cells and 10ug of undiluted antibody.

Mr. Dan Hasson

Verified customer

Submitted Jan 15 2018

Western blot
Human Cell lysate - nuclear (K562 erythroleukemia cell line)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Loading amount
10000 cells
K562 erythroleukemia cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

David Valle-Garcia

Verified customer

Submitted May 09 2016

Human Cell lysate - nuclear (K562 erythroleukemia cell line)
Negative control
ZNF358 / ATRX KO cell line
K562 erythroleukemia cell line
Detection step
Real-time PCR
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control
ZNF180, ZNF268, ZNF700, ZNF721

David Valle-Garcia

Verified customer

Submitted Apr 14 2016

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