Key features and details
- Rabbit polyclonal to ATRX
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ATRX antibody
See all ATRX primary antibodies
DescriptionRabbit polyclonal to ATRX
Tested applicationsSuitable for: IP, WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human ATRX aa 2211-2413.
Database link: P46100
- 293T, HepG2, Raji, NIH-3T3, HeLa A431, H1299 and HeLaS3 cell lines. A549 xenograft.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 283 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells which probably implies recruitment of CBX5 to telomers. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting; according to a report, it is not sufficient to decrease chromatin condensation at telomers nor to increase expression of telomeric RNA in fibroblasts (PubMed:24500201). May be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. Acts as negative regulator of chromatin incorporation of transcriptionally repressive histone H2AFY, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. May be involved in brain development and facial morphogenesis. Binds to zinc-finger coding genes with atypical chromatin signatures and regulates its H3K9me3 levels. Forms a complex with ZNF274, TRIM28 and SETDB1 to facilitate the deposition and maintenance of H3K9me3 at the 3' exons of zinc-finger genes (PubMed:27029610).
Involvement in diseaseAlpha-thalassemia mental retardation syndrome, X-linked
Mental retardation, X-linked, syndromic, with hypotonic facies 1
Alpha-thalassemia myelodysplasia syndrome
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 PHD-type zinc finger.
DomainThe ADD domain predominantly interacts with histone H3 trimethylated at 'Lys-10'(H3K9me3) (and to a lesser extent H3 mono-or dimethylated at 'Lys-10') and simultanously to histone H3 unmethylated at 'Lys-5' (H3K4me0). The interaction with H3K9me3 is disrupted by the presence of H3K4me3 suggesting a readout of the combined histone H3 methylation state.
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
modificationsPhosphorylated at serine residues during mitose. Phosphorylation may promote the release from the nuclear matrix and progression to mitosis.
Cellular localizationNucleus. Chromosome, telomere. Nucleus, PML body. Associated with pericentromeric heterochromatin during interphase and mitosis, probably by interacting with CBX5/HP1 alpha. Colocalizes with histone H3.3, DAXX, HIRA and ASF1A at PML-nuclear bodies. Colocalizes with cohesin (SMC1 and SMC3) and MECP2 at the maternal H19 ICR (By similarity).
- Information by UniProt
- Alpha thalassemia/mental retardation syndrome X linked homolog antibody
- ATP dependent helicase ATRX antibody
- ATP-dependent helicase ATRX antibody
Western blot of (lane1) PC12 (brain)whole cell extracts, (lane 2) Rat 2 (embryo) whole cell extracts, at 30µg per lane, labeling ATRX with ab97508 at 1:1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma labeling ATRX with ab97508 at 1/500 dilution. Anti-ATRX antibody (ab97508) detects ATRX protein at nucleus on human breast carcinoma tissue sections.
All lanes : Anti-ATRX antibody (ab97508) at 1/500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 283 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of endometrial carcinoma labeling ATRX with ab97508 at 1/500 dilution. Anti-ATRX antibody (ab97508) detects ATRX protein at nucleus on human endometrial carcinoma tissue sections.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma,using ab97508 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence analysis of mouse brain labeling ATRX with ab97508 at 1/100 dilution. Left- DAPI (blue), middle - ATRX protein staining (red), right - merge.
ab97508 at 5µg immunoprecipitating ATRX in 293T whole cell extracts.
Lane 1 (input): 293T whole cell extracts.
Lane 2 (-): Rabbit IgG instead of ab97508 in 293T whole cell extracts.
Lane 3 (+): Anti-ATRX antibody (ab97508) + 293T whole cell extracts.
Anti-ATRX antibody (ab97508) at 1/1000 dilution + NIH3T3 whole cell extracts at 30 µg
Predicted band size: 283 kDa
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97508 at 1/200 dilution. Lower image is merged with DNA probe.
Anti-ATRX antibody (ab97508) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 283 kDa
ab97508 has been referenced in 18 publications.
- Aschacher T et al. Long interspersed element-1 ribonucleoprotein particles protect telomeric ends in alternative lengthening of telomeres dependent cells. Neoplasia 22:61-75 (2020). PubMed: 31846834
- Lovejoy CA et al. ATRX affects the repair of telomeric DSBs by promoting cohesion and a DAXX-dependent activity. PLoS Biol 18:e3000594 (2020). PubMed: 31895940
- Suzuki H et al. Molecular Aberrations Associated with Seizure Control in Diffuse Astrocytic and Oligodendroglial Tumors. Neurol Med Chir (Tokyo) 60:147-155 (2020). PubMed: 32009124
- Cavalcante SG et al. ATRX-DAXX Complex Expression Levels and Telomere Length in Normal Young and Elder Autopsy Human Brains. DNA Cell Biol 38:955-961 (2019). PubMed: 31361513
- Merkl PE & Knipe DM Role for a Filamentous Nuclear Assembly of IFI16, DNA, and Host Factors in Restriction of Herpesviral Infection. MBio 10:N/A (2019). PubMed: 30670617