Product nameAtto 488 Conjugation Kit - Lightning-Link®
This product is manufactured by Expedeon, an Abcam company. Expedeon product code 350-0010 was previously called Lightning-Link® Rapid Atto 488 Labeling Kit 3 reactions each up to 200 µg and is the same as the 3 x 100 µg size of this product. Expedeon product code 350-0030 was previously called Lightning-Link® Rapid Atto 488 Labeling Kit 3 reactions each up to 20 µg and is the same as the 3 x 10 µg size of this product. Expedeon product code 350-0005 was previously called Lightning-Link® Rapid Atto 488 Labeling Kit 1 reaction up to 200 µg and is the same as the 100 µg size of this product. Expedeon product code 350-0015 was previously called Lightning-Link® Rapid Atto 488 Labeling Kit 1 reaction up to 2 mg and is the same as the 1 mg size of this product.
Atto 488 Conjugation Kit / Atto 488 Labeling Kit (ab269896) uses a simple and quick process for Atto 488 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.
To conjugate an antibody to Atto 488 using this kit:
- add modifier to antibody and incubate for 15 mins
- add quencher and incubate for 5 mins
The Atto 488 conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to Atto 488.
Amount and volume of antibody for conjugation to Atto 488
Kit size Recommended
amount of antibody1
amount of antibody
30 µg 3 x 10 µg 3 x 20 µg 3 x 10 µL 100 µg 100 µg 200 µg 100 µL 300 µg 3 x 100 µg 3 x 200 µg 3 x 100 µL 1 mg 1 mg 2 mg 1 mL
1 Using the maximum amount of antibody may result in less labeling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
50mM / 0.6% Tris1 0.1% BSA2 50% glycerol 0.1% sodium azide PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
Thiomerosal Proclin Glycine Arginine Glutathione DTT
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 µg 1 mg 3 x 10 µg 3 x 100 µg Atto 488 mix (Lyophilized) 1 vial 1 vial 3 vials 3 vials Modifier reagent 1 vial 1 vial 1 vial 1 vial Quencher reagent 1 vial 1 vial 1 vial 1 vial
Olling A et al. used ab269896 as part of examining the role of toxins in the pathogenicity of Clostridium difficile.
They used the kit to conjugate Atto 488 to TcdA1–1874 toxin for use in flow cytometry.
Binding of fluorescent labeled TcdA-PE/Cy5 and TcdA1–1874-Atto488 to HT29 and CHO-C6 cells was investigated by FACS analysis. Right shift of the black curve illustrates toxin binding which was detected through fluorescence emission at 667 nm for TcdA and at 523 nm for TcdA1–1874, respectively. Due to different ratio of fluorophor and toxin, fluorescence intensity of TcdA-PE/Cy5 cannot directly be compared with TcdA1–1874-Atto488.
Zeinolabediny Y et al. used ab269896 as part of examining the toxicity of p17 protein assemblies.
They used the kit to conjugate Atto 488 to p17 protein for use in microscopy.
Representative images of the p17 localisation as overlays of Atto 488-fluorescence and light microscopy in worms.
Scale bar = 50 µm. Worms fed for 2 h vehicle (10 mM PB, pH 7.4) or 4 nM p17-Atto 488.
ab269896 has not yet been referenced specifically in any publications.