Product nameAtto 565 Conjugation Kit - Lightning-Link®
This product is manufactured by Expedeon, an Abcam company. Expedeon product code 351-0010 was previously called Lightning-Link® Rapid Atto 565 Labeling Kit 3 reactions each up to 200 µg and is the same as the 3 x 100 µg size of this product. Expedeon product code 351-0030 was previously called Lightning-Link® Rapid Atto 565 Labeling Kit 3 reactions each up to 20 µg and is the same as the 3 x 10 µg size of this product. Expedeon product code 351-0005 was previously called Lightning-Link® Rapid Atto 565 Labeling Kit 1 reaction up to 200 µg and is the same as the 100 µg size of this product.
Atto 565 Conjugation Kit - Lightning-Link® (ab269897) provides an easy-to-use, one step procedure that allows researchers to covalently label proteins, peptides and other biomolecules containing primary amines with Atto 565 with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.
The antibody to be labeled should be purified, in an appropriate buffer for conjugation and at a suitable concentration.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to Atto 565.
Amount and volume of antibody for conjugation to Atto 565
Kit size Recommended
amount of antibody1
amount of antibody
30 µg 3 x 10 µg 3 x 20 µg 3 x 10 µL 100 µg 100 µg 200 µg 100 µL 300 µg 3 x 100 µg 3 x 200 µg 3 x 100 µL
1 Using the maximum amount of antibody may result in less labeling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
50mM / 0.6% Tris1 0.1% BSA2 50% glycerol 0.1% sodium azide PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
Thiomerosal Proclin Glycine Arginine Glutathione DTT
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 µg 3 x 10 µg 3 x 100 µg Atto 565 mix (Lyophilized) 1 vial 3 vials 3 vials Modifier reagent 1 vial 1 vial 1 vial Quencher reagent 1 vial 1 vial 1 vial
ab269897 has not yet been referenced specifically in any publications.