Key features and details
- Rabbit polyclonal to ATTY
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATTY antibody
See all ATTY primary antibodies
DescriptionRabbit polyclonal to ATTY
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Zebrafish
Synthetic peptide corresponding to Human ATTY aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: NIH3T3; HepG2; PC12; A20; Caco2; SKNBE; MCF7; K562; Raji; A431.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab91450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).|
FunctionTransaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has no transaminase activity towards phenylalanine.
PathwayAmino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 2/6.
Involvement in diseaseDefects in TAT are the cause of tyrosinemia type 2 (TYRO2) [MIM:276600]; also known as Richner-Hanhart syndrome. TYRO2 is an inborn error of metabolism characterized by elevations of tyrosine in the blood and urine, and oculocutaneous manifestations. Typical features include palmoplantar keratosis, painful corneal ulcers, and mental retardation.
Sequence similaritiesBelongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
- Information by UniProt
- ATTY_HUMAN antibody
- L-tyrosine:2-oxoglutarate aminotransferase antibody
- TAT antibody
All lanes : Anti-ATTY antibody (ab91450) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 :
A20 whole cell lysate (ab7180)
Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 6 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 8 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 9 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 10 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
ICC/IF image of ab91450 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91450, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab91450 has not yet been referenced specifically in any publications.