Key features and details
- Rabbit polyclonal to ATXN2L
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATXN2L antibody
See all ATXN2L primary antibodies
DescriptionRabbit polyclonal to ATXN2L
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide within Human ATXN2L aa 150-200. The exact sequence is proprietary.
Database link: NP_009176.2
- WB: HeLa and 293T whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 114 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
Tissue specificityExpressed at high levels in thymus, lymph node, spleen, fetal kidney and adult testis. Constitutively associated with MPL and EPOR in hematopoietic cells.
Sequence similaritiesBelongs to the ataxin-2 family.
modificationsThrombopoietin triggers the phosphorylation on tyrosine residues in a way that is dependent on MPL C-terminal domain.
Arg-361 is dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- A2D antibody
- A2LG antibody
- A2LP antibody
All lanes : Anti-ATXN2L antibody (ab99304) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Exposure time: 30 seconds
ab99304, at 0.1 µg/ml, staining ATXN2L in HeLa whole cell lysate immunoprecipitated using ab99304 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.
ab99304 has not yet been referenced specifically in any publications.