Overview

  • Product name

  • Description

    Rabbit polyclonal to ATXN2L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide within Human ATXN2L aa 150-200. The exact sequence is proprietary.
    Database link: NP_009176.2

  • Positive control

    • WB: HeLa and 293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab99304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Tissue specificity

    Expressed at high levels in thymus, lymph node, spleen, fetal kidney and adult testis. Constitutively associated with MPL and EPOR in hematopoietic cells.
  • Sequence similarities

    Belongs to the ataxin-2 family.
  • Post-translational
    modifications

    Thrombopoietin triggers the phosphorylation on tyrosine residues in a way that is dependent on MPL C-terminal domain.
    Arg-361 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A2D antibody
    • A2LG antibody
    • A2LP antibody
    • A2RP antibody
    • Ataxin 2 domain protein antibody
    • Ataxin 2 like antibody
    • Ataxin 2 like protein antibody
    • Ataxin 2 related protein antibody
    • Ataxin-2 domain protein antibody
    • Ataxin-2 like antibody
    • Ataxin-2-like protein antibody
    • Ataxin-2-related protein antibody
    • ATX2L_HUMAN antibody
    • ATXN2L antibody
    • ATXN2L ataxin 2 like antibody
    see all

Images

  • All lanes : Anti-ATXN2L antibody (ab99304) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 114 kDa


    Exposure time: 30 seconds
  • ab99304, at 0.1 µg/ml, staining ATXN2L in HeLa whole cell lysate immunoprecipitated using ab99304 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab99304 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you very much for your interest our AbTrial program.

While ab99304 has not been tested for use on mouse samples, the immunogen is 100% homologous with the mouse sequence. With our AbTrial program, you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

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