Product nameAnti-ATXN2L antibody
See all ATXN2L primary antibodies
DescriptionRabbit polyclonal to ATXN2L
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide within Human ATXN2L aa 150-200. The exact sequence is proprietary.
Database link: NP_009176.2
- WB: HeLa and 293T whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 114 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
Tissue specificityExpressed at high levels in thymus, lymph node, spleen, fetal kidney and adult testis. Constitutively associated with MPL and EPOR in hematopoietic cells.
Sequence similaritiesBelongs to the ataxin-2 family.
modificationsThrombopoietin triggers the phosphorylation on tyrosine residues in a way that is dependent on MPL C-terminal domain.
Arg-361 is dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- A2D antibody
- A2LG antibody
- A2LP antibody
All lanes : Anti-ATXN2L antibody (ab99304) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Exposure time: 30 seconds
ab99304, at 0.1 µg/ml, staining ATXN2L in HeLa whole cell lysate immunoprecipitated using ab99304 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.
ab99304 has not yet been referenced specifically in any publications.