Overview

  • Product name

    Anti-AUH antibody [EPR11086(B)]
    See all AUH primary antibodies
  • Description

    Rabbit monoclonal [EPR11086(B)] to AUH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human AUH (Uniprot: Q13825).

  • Positive control

    • Mouse brain, Rat brain, Rat heart and Rat kidney cell lysates; SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Relevance

      AUH (3-methylglutaconyl-CoA hydratase) catalyzes the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA and has very low enoyl-CoA hydratase activity. Deletion or mutation of the AUH gene causes the metabolic disease 3-methylglutaconic aciduria type I (MGA1). MGA type I is characterized by an abnormal organic acid profile in which there is excessive urinary excretion of 3-methylglutaconic acid, 3-methylglutaric acid and 3-hydroxyisovaleric acid. AUH is also an RNA-binding protein that binds in vitro to clustered 5'-AUUUA-3' motifs.
    • Cellular localization

      Mitochondrial
    • Database links

    • Alternative names

      • 3 methylglutaconyl CoA hydratase antibody
      • AU binding protein/enoyl CoA hydratase antibody
      • AU RNA binding protein/enoyl CoA hydratase antibody
      • AU RNA binding protein/enoyl Coenzyme A hydratase antibody
      • AU specific RNA binding antibody
      • AU specific RNA binding enoyl CoA hydratase antibody
      • Methylglutaconyl CoA hydratase antibody
      • Methylglutaconyl CoA hydratase mitochondrial antibody
      • MGA Type I antibody
      see all

    Images

    • All lanes : Anti-AUH antibody [EPR11086(B)] (ab157453) at 1/1000 dilution

      Lane 1 : Mouse brain cell lysate
      Lane 2 : Rat brain cell lysate
      Lane 3 : Rat heart cell lysate
      Lane 4 : Rat kidney cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 36 kDa

    • Immunofluorescent analysis of SH-SY5Y cells labeling AUH with ab157453 at 1/100 dilution.

    References

    This product has been referenced in:

    See 1 Publication for this product

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