Recombinant
RabMAb

Anti-AUH antibody [EPR11087(B)] (ab155980)

Overview

  • Product name
    Anti-AUH antibody [EPR11087(B)]
    See all AUH primary antibodies
  • Description
    Rabbit monoclonal [EPR11087(B)] to AUH
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human AUH isoform 2 (UniProt ID: Q13825-2).

  • Positive control
    • HepG2 and SH SY5Y cell lysates; Human fetal kidney lysate; Human pancreas tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab155980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Relevance
      AUH (3-methylglutaconyl-CoA hydratase) catalyzes the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA and has very low enoyl-CoA hydratase activity. Deletion or mutation of the AUH gene causes the metabolic disease 3-methylglutaconic aciduria type I (MGA1). MGA type I is characterized by an abnormal organic acid profile in which there is excessive urinary excretion of 3-methylglutaconic acid, 3-methylglutaric acid and 3-hydroxyisovaleric acid. AUH is also an RNA-binding protein that binds in vitro to clustered 5'-AUUUA-3' motifs.
    • Cellular localization
      Mitochondrial
    • Database links
    • Alternative names
      • 3 methylglutaconyl CoA hydratase antibody
      • AU binding protein/enoyl CoA hydratase antibody
      • AU RNA binding protein/enoyl CoA hydratase antibody
      • AU RNA binding protein/enoyl Coenzyme A hydratase antibody
      • AU specific RNA binding antibody
      • AU specific RNA binding enoyl CoA hydratase antibody
      • Methylglutaconyl CoA hydratase antibody
      • Methylglutaconyl CoA hydratase mitochondrial antibody
      • MGA Type I antibody
      see all

    Images

    • ab155980 showing +ve staining in Human thyroid gland carcinoma.
    • ab155980 showing +ve staining in Human normal kidney.
    • ab155980 showing +ve staining in Human normal heart.
    • ab155980 showing +ve staining in Human endometrial carcinoma.
    • ab155980 showing +ve staining in Human normal brain.
    • All lanes : Anti-AUH antibody [EPR11087(B)] (ab155980) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SH SY5Y lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 32 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling AUH using ab155980 at a 1/50 dilution.

    References

    ab155980 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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