Recombinant
RabMAb

Recombinant Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (ab215823)

Overview

  • Product name

    Anti-AUH antibody [EPR11087(B)] - BSA and Azide free
    See all AUH primary antibodies
  • Description

    Rabbit monoclonal [EPR11087(B)] to AUH - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human AUH. The exact sequence is proprietary. Corresponding to residues in Human AUH isoform 2.
    Database link: Q13825-2

  • Positive control

    • HepG2 and SH SY5Y cell lysates; Human fetal kidney lysate; Human pancreas tissue.
  • General notes

    Ab215823 is the carrier-free version of ab155980. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab215823 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Relevance

      AUH (3-methylglutaconyl-CoA hydratase) catalyzes the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA and has very low enoyl-CoA hydratase activity. Deletion or mutation of the AUH gene causes the metabolic disease 3-methylglutaconic aciduria type I (MGA1). MGA type I is characterized by an abnormal organic acid profile in which there is excessive urinary excretion of 3-methylglutaconic acid, 3-methylglutaric acid and 3-hydroxyisovaleric acid. AUH is also an RNA-binding protein that binds in vitro to clustered 5'-AUUUA-3' motifs.
    • Cellular localization

      Mitochondrial
    • Database links

    • Alternative names

      • 3 methylglutaconyl CoA hydratase antibody
      • AU binding protein/enoyl CoA hydratase antibody
      • AU RNA binding protein/enoyl CoA hydratase antibody
      • AU RNA binding protein/enoyl Coenzyme A hydratase antibody
      • AU specific RNA binding antibody
      • AU specific RNA binding enoyl CoA hydratase antibody
      • Methylglutaconyl CoA hydratase antibody
      • Methylglutaconyl CoA hydratase mitochondrial antibody
      • MGA Type I antibody
      see all

    References

    ab215823 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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