Overview

  • Product name
  • Description
    Rabbit polyclonal to Aurora A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Serine/Threonine Kinase 15 encoded within exon 5 (LocusLink ID 8465)

Properties

Applications

Our Abpromise guarantee covers the use of ab1287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/1000 for 1 hr on HeLa cells (see Abreview).
IHC-P 1/250 - 1/1000.
WB 1/4000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast, pancreas and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly after.
IP Use a concentration of 2 - 10 µg/ml.

Target

  • Function
    Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
  • Tissue specificity
    Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIK antibody
    • ARK-1 antibody
    • ARK1 antibody
    • AURA antibody
    • Aurka antibody
    • Aurora 2 antibody
    • Aurora A antibody
    • Aurora kinase A antibody
    • Aurora-related kinase 1 antibody
    • Aurora/IPL1 like kinase antibody
    • Aurora/IPL1-related kinase 1 antibody
    • AURORA2 antibody
    • Breast tumor-amplified kinase antibody
    • BTAK antibody
    • hARK1 antibody
    • IAK antibody
    • IPL1 related kinase antibody
    • MGC34538 antibody
    • OTTHUMP00000031340 antibody
    • OTTHUMP00000031341 antibody
    • OTTHUMP00000031342 antibody
    • OTTHUMP00000031343 antibody
    • OTTHUMP00000031344 antibody
    • OTTHUMP00000031345 antibody
    • OTTHUMP00000166071 antibody
    • OTTHUMP00000166072 antibody
    • PPP1R47 antibody
    • Protein phosphatase 1, regulatory subunit 47 antibody
    • Serine/threonine kinase 15 antibody
    • Serine/threonine kinase 6 antibody
    • Serine/threonine-protein kinase 15 antibody
    • Serine/threonine-protein kinase 6 antibody
    • Serine/threonine-protein kinase aurora-A antibody
    • STK15 antibody
    • STK6 antibody
    • STK6_HUMAN antibody
    • STK7 antibody
    see all

Images

  • Immunoprecipitation of Human Aurora A.
    35S-Met labeled whole cell lysate from cells transfected with a human Aurora A expression construct. ab1287 was used at 4 µg/ml for IP. Detection: Autoradiography.
  • ab1287 (1/250) detecting Aurora A in paraffin embedded Human pancreatic tumour tissue sections. Detection: DAB staining;
  • ab1287 at a 1/1000 dilution staining synchronised HeLa cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 1% serum prior to incubation with the primary antibody for 1 hour. A Cy5 conjugated donkey anti-rabbit antibody was used as the secondary. The image shows a DAPI panel, Aurora-A panel, and merge panel of DAPI (blue), microtubules (red) and Aurora-A (green).

    See Abreview

  • Anti-Aurora A antibody (ab1287) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa
    Additional bands at: 20 kDa, 25 kDa, 28 kDa, 37 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Grant R  et al. Constitutive regulation of mitochondrial morphology by Aurora A kinase depends on a predicted cryptic targeting sequence at the N-terminus. Open Biol 8:N/A (2018). Read more (PubMed: 29899121) »
  • Ma Y  et al. Aurora-A affects radiosenstivity in cervical squamous cell carcinoma and predicts poor prognosis. Oncotarget 8:31509-31520 (2017). Read more (PubMed: 28404933) »
See all 25 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

In general, we recommend the tissue you see on the datasheet, in this case a pancreatic tumor. We offer sections of pancreatic adenocarcinoma, not of the exact same tumor, but we assume the staining will be similar. Here is a link to the datasheet for the sections.

https://www.abcam.com/pancreas-tumor-human-adenocarcinoma-tissue-slides-ab5036.html

I also recommend consulting the Human Protein Atlas. The entry for Aurora A is at these links:

http://www.proteinatlas.org/ENSG00000087586/tissue

http://www.proteinatlas.org/ENSG00000087586/tissue/staining+overview

The current data is from only two antibodies, though.

For normal tissue, the UniProt entry for human Aurora A (http://www.uniprot.org/uniprot/O14965) has this note, but without a reference.

Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.

We have sections of many paraffin-embedded tissues, including testis, which are easy to find by typing the name of the tissue in the search box on our website and filtering the list of results by product type.

Read More

Answer

Thank you for your enquiry and your interest in our products.

I can confirm that we have 9 vials of batch: GR4766-7 in our UK stock.

I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

Question

BATCH NUMBER 165680 ORDER NUMBER 164559 DESCRIPTION OF THE PROBLEM enquiry ref: 889878 This package was simple, only used one small ice bag ANTIBODY STORAGE CONDITIONS The Abs were in RT when we received ADDITIONAL NOTES As we haven't positive control slide for the Aurora A, and we are very busy, we didn't do IHC, but we did Western Blotting for all these four abs that we ordered and you shipped together, we don't know if this Aurora A is working or not, but the ER-a is not working, so we ask replace Aurora A too. Attached is Western Blotting results by JPE format, if you can't open please let me know Following are my more detail standpoints. The Abs were definitely in RT when we received This package was simple, only used one small ice bag (around half pond). If you still persist in, your technique department can simulate this shipment, see what will go on. We paid shipping fee, inside are thousand dollar worth four Abs, abcam should be responsible for the Abs¡¯ quality using correct ice package.This was two days and hot summer shipment, we don¡¯t know what UPS ¡® Delivery status was, especially on rigorous delivery truck.Abs¡¯ properties are very as you know, even unhappy with us when received The four Abs, we still set a Western Blotting test at 2nd day: Cyclin b1 and PCNA birth have correct bands, ER-a had not any band and we haven¡¯ IHC positive control slide for Aurora AWe bought in bulk mount of Abs from abcam or others every year, we have lots of experiments every week, we are very busy, hopefully, this is last enquiry on this case. Your understanding is my pleasure!

Read More
Answer

Thank you for your enquiry. I am afraid your image did not come through. All of our antibodies are stable at room temperature for at least 2 weeks. From the information you have given, I cannot tell whether there may be something in your protocol that can be optimized for better results, or if the antibody is really at fault. If you could fill out the form again with the details of your Western blot protocol, I can look into this further. Please find the link again below for your convenience. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=1287&mode=questionaire I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%

Mr. Michael Porter

Verified customer

Submitted Aug 14 2006

Answer

Thank you for your enquiry. For ab1287, the originator has recommended using 0.1 M citrate buffer, pH 6.6 and 1mM EDTA. For ab2254, we would suggest microwave pre-treatment in citrate buffer (pH 6.0) for 5 mins high then 5 mins simmer (800W conventional microwave). If you have any additional questions, please contact us again.

Read More

Answer

I'm afraid the precise immunogen sequence used is proprietary. The binding site is between residues 50 and 100 of human Aurora A using the numbering given in entry NP_003591.1. I hope this information helps,

Read More

Answer

Thank you for your email. The immunogen used was a synthetic peptide (Human) conjugated to KLH - which represented a portion of human Serine/Threonine Kinase 15 encoded within exon 5 (LocusLink ID 8465). If you have any more questions, please contact us again.

Read More

Answer

Thank you for your enquiry. The buffer is 50 mM Tris, 5 mM Citrate and 40 mM Phosphate. If you need any further assistance please do not hesitate to get back in touch.

Read More

Answer

Thank you for your enquiry and for sending us your detailed protocol. We would suggest blocking the non-specific binding sites with 1%BSA for 1 hr and incubating the samples with the primary antibody for 2 hrs (40 min is too short). This antibody ahs been tested for IHC using human pancreatic tumor sections, so it may be worth using this type of tissue sections as positive control. Should you still have problem with this antibody, please do not hesitate to contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up