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My technician forwarded your note below to me and I simply do not understand your response. You say that the antibody noted below is the best bet as the peptide has a fair amount of alignment between human and Chlamy Aurora A. Well, although the two proteins have 67% homology, there is no overlap at all that I can detect between the human peptide immunogen and any part of the Chlamy Aurora A sequence.
Have you made a mistake, or posted an incorrect ab link? I am at a loss for understanding your suggested ab (and if I hadn't looked, I would be at a loss for about $*** as well).
Asked on Dec 04 2012
Thank you for your reply.
I apologize for the confusion, there was a difference in the numerical order of amino acids listed in the alignment I was performing. You are correct, ab115883 should not be chosen as the immunogen sequence does not overlap with the Chlamy Aurora A sequence.
I reran the search using the proper numerical organization and came up with the following antibody:
ab61114 (https://www.abcam.com/Aurora-A-antibody-ab61114.html): the immunogen sequence is around the human Aurora A amino acid 288. The human and Chlamy sequences are quite homologous in this region, as amino acids 285 and 288 are the only two mismatched amino acids from 275-301.
I'm actually confident enough in the homology within this region to offer our Abtrial program for testing ab61114 in your Chlamy sequences. Information regarding this program is given below:
To our knowledge, ab61114 has not been tested in C. reinhardtii. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.
Simply follow these easy steps below to apply for our AbTrial Program:
1. Reply to this email, letting us know you are interested in testing this product.
2. Our scientists will email you an inactive personal discount code for the value of the product.
3. Purchase and test the product at the regular price.
4. Submit your results, including your discount code in the additional notes section of your Abreview.
5. Once the Abreview is submitted, the discount code will become active.
6. Apply your discount code on your next order to receive that value off.
The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial
Additionally, until 12/16/12 we are running a rabbit monoclonal antibody promotion, our "RabMab" promotion, which entitles you to a free rabbit monoclonal antibody with purchase of a primary antibody. I found a rabbit monoclonal antibody ab108353 (https://www.abcam.com/Aurora-A-antibody-EPR5026-ab108353.html) that has 54% homology with the Chlamy sequence and might be worth a try, otherwise you could choose a different Rabbit monoclonal antibody that you desire. Details of the RabMab promotion are given below:
Please let me know if you have any questions about this offer and I would be happy to help you further.
Answered on Dec 05 2012