Immunocytochemistry abreview for Anti-Aurora A antibody

Average
Abreviews
Application
Immunocytochemistry
Sample
Human Cell (HeLa and SK-N-SH)
Specification
HeLa and SK-N-SH
Fixative
Paraformaldehyde

Other product details

Dilution
1/500
Incubation time
30 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®

Additional data

Additional Notes
I tested this antibody in paraformaldehyde-fixed HeLa and SK-N-SH cells and it appears to only recognize the Aurora A epitope in HeLa. It did not recognize Aurora A in SK-N-SH at 1:100, 1:200 or 1:500 dilutions.

In HeLa this antibody gives a centrosomal staining pattern from late G2 until metaphase where is appears to localize to the midbody/cleavage furrow.

Dr. Kirk Mcmanus

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Submitted Oct 12 2005

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