I tested this antibody in paraformaldehyde-fixed HeLa and SK-N-SH cells and it appears to only recognize the Aurora A epitope in HeLa. It did not recognize Aurora A in SK-N-SH at 1:100, 1:200 or 1:500 dilutions.
In HeLa this antibody gives a centrosomal staining pattern from late G2 until metaphase where is appears to localize to the midbody/cleavage furrow.
Dr. Kirk Mcmanus
Submitted Oct 12 2005
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