Western blot abreview for Anti-Aurora A antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
20 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 15°C

Other product details

Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit conjugated HRP secondary antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL amersham
Exposure
30 second(s)
Bands
Specific: 40 kDa
Positive control
control HeLa cells
Negative control
transfection of siRNA specific to Aurora kinase A

Ms. AeLee Jeong

Verified customer

Submitted Apr 04 2013

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