Western blot abreview for Anti-Aurora A antibody - Centrosome Marker

Inconclusive
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Sample
Human Cell lysate - whole cell (U2OS-osteosarcoma)
Specification
U2OS-osteosarcoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-0.1%tween20-1%BSA
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: goat-anti-rabbit-HRP
Host species: Human
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 50 kDa Non-specific: 110, 100, 72, 68 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
siRNA against luciferase
Positive control
siRNA against Aurora-A

Additional data

Abcam response
Thank you for your valuable feedback! This antibody does not seem to work well under these experimental conditions. A shorter incubation time with the primary antibody and more stringent washes might help with the trouble shooting. Thank you for your time - we hope that this information will be helpful for future customers.

Dr. Carlos le Sage

Verified customer

Submitted Jan 21 2014

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