Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aurora A antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Bladder)
Specification
Bladder
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Other product details

Dilution
1/10
Incubation time
15 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: biotinylated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
HIER - slides microwaved at full power in citrate buffer (pH6.0) for 3 mins, left to cool for 5 mins and microwaved for a further 3mins.
Blocking - 10% normal goat serum.
Primary - incubated overnight at 4 degrees centigrade.

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Submitted Jan 23 2007

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