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  1. Link

    aurora-a-antibody-epr5026-centrosome-marker-ab108353.pdf

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RecombinantRabMAb

Recombinant Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)

  • Datasheet
  • SDS
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Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
  • Immunocytochemistry/ Immunofluorescence - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
  • Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
  • Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
  • Flow Cytometry (Intracellular) - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
  • Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
  • OI-RD Scanning - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
  • Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5026] to Aurora A - Centrosome Marker
  • Suitable for: WB, ICC/IF, IP, Flow Cyt (Intra)
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-Aurora A antibody [EPR5026] - Centrosome Marker
    See all Aurora A primary antibodies
  • Description

    Rabbit monoclonal [EPR5026] to Aurora A - Centrosome Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: BXPC-3, LnCaP, SKBR-3 and HepG2 cell lysates. ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.63 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5026
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Aurora
    • Cancer
    • Cell cycle
    • Cell division
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Alternative Versions

    • Anti-Aurora A antibody [EPR5026] - BSA and Azide free (ab247643)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Aurora A protein (ab42595)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
ICC/IF
1/100 - 1/250.
IP
1/60.
Flow Cyt (Intra)
1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
ICC/IF
1/100 - 1/250.
IP
1/60.
Flow Cyt (Intra)
1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
  • Tissue specificity

    Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
  • Target information above from: UniProt accession O14965 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6790 Human
    • Entrez Gene: 20878 Mouse
    • Omim: 603072 Human
    • SwissProt: O14965 Human
    • SwissProt: P97477 Mouse
    • Unigene: 250822 Human
    • Unigene: 249363 Mouse
    • Alternative names

      • AIK antibody
      • ARK-1 antibody
      • ARK1 antibody
      • AURA antibody
      • Aurka antibody
      • Aurora 2 antibody
      • Aurora A antibody
      • Aurora kinase A antibody
      • Aurora-related kinase 1 antibody
      • Aurora/IPL1 like kinase antibody
      • AURORA/IPL1-like kinase antibody
      • Aurora/IPL1-related kinase 1 antibody
      • AURORA2 antibody
      • Breast tumor-amplified kinase antibody
      • BTAK antibody
      • hARK1 antibody
      • IAK antibody
      • IPL1 related kinase antibody
      • MGC34538 antibody
      • OTTHUMP00000031340 antibody
      • OTTHUMP00000031341 antibody
      • OTTHUMP00000031342 antibody
      • OTTHUMP00000031343 antibody
      • OTTHUMP00000031344 antibody
      • OTTHUMP00000031345 antibody
      • OTTHUMP00000166071 antibody
      • OTTHUMP00000166072 antibody
      • PPP1R47 antibody
      • Protein phosphatase 1, regulatory subunit 47 antibody
      • Serine/threonine kinase 15 antibody
      • Serine/threonine kinase 6 antibody
      • Serine/threonine protein kinase 15 antibody
      • Serine/threonine-protein kinase 15 antibody
      • Serine/threonine-protein kinase 6 antibody
      • Serine/threonine-protein kinase aurora-A antibody
      • STK15 antibody
      • STK6 antibody
      • STK6_HUMAN antibody
      • STK7 antibody
      see all

    Images

    • Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
      Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
      All lanes : Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353) at 1/1000 dilution

      Lane 1 : BXPC-3 cell lysate
      Lane 2 : LnCaP cell lysate
      Lane 3 : SKBR-3 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 46 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
      Immunocytochemistry/ Immunofluorescence - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
      Immunofluorescent staining of HeLa cells using ab108353 at 1/100
    • Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
      Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)

      Lane 1: Neuro2a (Mouse neuroblastoma neuroblast) whole cell lysate, 10µg
      Lane 2:
      Neuro2a cell lysate 350µg and ab109518 2µg
      Lane 3:
      Neuro2a cell lysate, 350µg and rabbit IgG (ab172730) , 2µg

      Purified ab108353 immunoprecipitating Aurora A in Neuro2a cell lysates. Primary antibody was used at a 1/60 dilution (20 µg/ml).  For western blotting, ab108353 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Blocking and diluting buffer used: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
      Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)

      Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
      Lane 2:
      HepG2 cell lysate 350µg and ab109518 2µg
      Lane 3:  
      HepG2 cell lysate 350µg and rabbit IgG (ab172730) , 2µg

      Purified ab108353 immunoprecipitating Aurora A in Neuro2a cell lysates. Primary antibody was used at a 1/60 dilution (20 µg/ml).  For western blotting, ab108353 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Blocking and diluting buffer used: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
      Flow Cytometry (Intracellular) - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)

      Overlay histogram showing HeLa cells stained with ab108353 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108353, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

       

    • Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
      Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
      Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353) at 1/1000 dilution + Neuro-2a cell lysate at 10 µg

      Predicted band size: 46 kDa

    • OI-RD Scanning - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
      OI-RD Scanning - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
      Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab108353? Please let us know so that we can cite the reference in this datasheet.

    ab108353 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Western blot abreview for Anti-Aurora A [EPR5026] antibody - Centrosome Marker

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris gel)
    Sample
    Human Cell lysate - whole cell (U2OS-osteosarcoma)
    Specification
    U2OS-osteosarcoma
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Carlos le Sage

    Verified customer

    Submitted Jan 21 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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