Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
Rabbit recombinant monoclonal Aurora A antibody [EPR5026]. Validated in WB, Flow Cyt, ICC/IF and tested in Mouse, Human. Independently reviewed in 1 review(s).
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Overview
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Product nameAnti-Aurora A antibody [EPR5026] - Centrosome Marker
See all Aurora A primary antibodies -
DescriptionRabbit monoclonal [EPR5026] to Aurora A - Centrosome Marker
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Host speciesRabbit
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Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide within Human Aurora A. The exact sequence is proprietary.
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Positive control
- BXPC-3, LnCaP, SKBR-3 and HepG2 cell lysates. HeLa cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 2.63 x 10 -10 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR5026
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab108353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 46 kDa. | |
| Flow Cyt | 1/1000 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/100 - 1/250. |
Target
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FunctionContributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
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Tissue specificityHighly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
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Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsActivated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome. -
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
- Information by UniProt
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Database links
- Entrez Gene: 6790 Human
- Entrez Gene: 20878 Mouse
- Omim: 603072 Human
- SwissProt: O14965 Human
- SwissProt: P97477 Mouse
- Unigene: 250822 Human
- Unigene: 249363 Mouse
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Alternative names
- AIK antibody
- ARK-1 antibody
- ARK1 antibody
see all
Images
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![Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)](https://www.abcam.com/ps/products/108/ab108353/Images/Aurora-A-Primary-antibodies-ab108353-1.jpg)
Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)All lanes : Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353) at 1/1000 dilution
Lane 1 : BXPC-3 cell lysate
Lane 2 : LnCaP cell lysate
Lane 3 : SKBR-3 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)](https://www.abcam.com/ps/products/108/ab108353/Images/Aurora-A-Primary-antibodies-ab108353-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)Immunofluorescent staining of HeLa cells using ab108353 at 1/100 -
![Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)](https://www.abcam.com/ps/products/108/ab108353/Images/ab108353-301067-ab108353IP1.jpg)
Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)Lane 1: Neuro2a (Mouse neuroblastoma neuroblast) whole cell lysate, 10µg
Lane 2: Neuro2a cell lysate 350µg and ab109518 2µg
Lane 3: Neuro2a cell lysate, 350µg and rabbit IgG (ab172730) , 2µgPurified ab108353 immunoprecipitating Aurora A in Neuro2a cell lysates. Primary antibody was used at a 1:500 dilution (2.2 µg/ml) . For western blotting, VeriBlot for IP (HRP) ab131366 was used as the secondary antibody at 1:1000 dilution.
Blocking and diluting buffer used: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)](https://www.abcam.com/ps/products/108/ab108353/Images/ab108353-301068-ab108353IP2.jpg)
Immunoprecipitation - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
Lane 2: HepG2 cell lysate 350µg and ab109518 2µg
Lane 3: HepG2 cell lysate 350µg and rabbit IgG (ab172730) , 2µg
Purified ab108353 immunoprecipitating Aurora A in Neuro2a cell lysates. Primary antibody was used at a 1:500 dilution (2.2 µg/ml). For western blotting, VeriBlot for IP (HRP) ab131366 was used as the secondary antibody at 1:1000 dilution.Blocking and diluting buffer used: 5% NFDM/TBST.
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![Flow Cytometry - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)](https://www.abcam.com/ps/products/108/ab108353/Images/ab108353-5-ab108353FC.jpg)
Flow Cytometry - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)Overlay histogram showing HeLa cells stained with ab108353 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108353, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)](https://www.abcam.com/ps/products/108/ab108353/Images/Aurora-A-Primary-antibodies-ab108353-3.jpg)
Western blot - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353) at 1/1000 dilution + Neuro-2a cell lysate at 10 µg
Predicted band size: 46 kDa -
![Other - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)](https://www.abcam.com/ps/products/108/ab108353/Images/Aurora-A-Primary-antibodies-ab108353-4.jpg)
Other - Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)
Protocols
Datasheets and documents
References
ab108353 has not yet been referenced specifically in any publications.
