Anti-Aurora A + B antibody [3H1] (ab190374)

Overview

  • Product name
    Anti-Aurora A + B antibody [3H1]
  • Description
    Mouse monoclonal [3H1] to Aurora A + B
  • Host species
    Mouse
  • Specificity
    ab190374 was tested for binding to expressed human Aurora A, B and C and shown to react with both Aurora A and B, but not C.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Chicken, Cow, Human, Pig
  • Immunogen

    Recombinant full length protein corresponding to Human Aurora A + B aa 1-403. purified from E. coli.
    Sequence:

    MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCP SNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQ PLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLA REKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYF HDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSK RVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEM IEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPD FVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESAS KQS


    Database link: O14965

  • Positive control
    • HeLa cells, treated with 100ng/ml nocodazole for 18 hours; recombinant Aurora A and B; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab190374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 38, 46 kDa.
ICC/IF 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin, and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between GSG2 and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes.
  • Cellular localization
    Nucleus. Chromosome. Chromosome › centromere. Cytoplasm › cytoskeleton › spindle. Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. Proper localization of the active, Thr-232-phosphorylated form during metaphase may be dependent upon interaction with SPDYC. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cytoplasm › cytoskeleton › spindle pole. Note: Detected at the neurite hillock in developing neurons
  • Database links
  • Alternative names
    • AIK antibody
    • AIK2 antibody
    • AIM-1 antibody
    • AIM1 antibody
    • AIRK1 antibody
    • AIRK2 antibody
    • ARK1 antibody
    • ARK2 antibody
    • AURA antibody
    • AURKA antibody
    • AURKB antibody
    • Aurora 1 antibody
    • Aurora 2 antibody
    • Aurora kinase A antibody
    • Aurora kinase B antibody
    • Aurora- and IPL1-like midbody-associated protein 1 antibody
    • Aurora-related kinase 1 antibody
    • Aurora-related kinase 2 antibody
    • Aurora/IPL1-related kinase 1 antibody
    • Aurora/IPL1-related kinase 2 antibody
    • AYK antibody
    • AYK1 antibody
    • Breast tumor-amplified kinase antibody
    • BTAK antibody
    • IAK1 antibody
    • Serine/threonine-protein kinase 12 antibody
    • Serine/threonine-protein kinase 15 antibody
    • Serine/threonine-protein kinase 5 antibody
    • Serine/threonine-protein kinase 6 antibody
    • Serine/threonine-protein kinase aurora-A antibody
    • Serine/threonine-protein kinase aurora-B antibody
    • STK-1 antibody
    • STK1 antibody
    • STK12 antibody
    • STK15 antibody
    • STK5 antibody
    • STK6 antibody
    see all

Images

  • Immunofluorescent analysis of HeLa cells labeling Aurora A + B with ab190374 at 1/500 dilution (green). Cells were counterstained with a polyclonal antibody to Vimentin (red). Blue is a DNA stain.

  • All lanes : Anti-Aurora A + B antibody [3H1] (ab190374) at 1/1000 dilution

    Lane 1 : HeLa cells treated with 100ng/ml nocodazole for 18 hours
    Lane 2 : Recombinant Aurora A
    Lane 3 : Recombinant Aurora B
    Lane 4 : Recombinant Aurora C

    Predicted band size: 38, 46 kDa

References

ab190374 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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