Anti-Aurora A (phospho T288) antibody (ab83968)
Key features and details
- Rabbit polyclonal to Aurora A (phospho T288)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Aurora A (phospho T288) antibody
See all Aurora A primary antibodies -
Description
Rabbit polyclonal to Aurora A (phospho T288) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Aurora A (phospho T288).
Database link: NP_003591.2 -
Positive control
- NBF fixed asynchronous HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Phospho Aurora A immobilized on a solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
1/100 - 1/500.
Formaldehyde fixed cells. Permeabilisation with Triton-X100 is recommended. |
Notes |
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ICC/IF
1/100 - 1/500. Formaldehyde fixed cells. Permeabilisation with Triton-X100 is recommended. |
Target
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Function
Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. -
Tissue specificity
Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsActivated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis. - Information by UniProt
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Database links
- Entrez Gene: 6790 Human
- Omim: 603072 Human
- SwissProt: O14965 Human
- Unigene: 250822 Human
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Alternative names
- AIK antibody
- ARK-1 antibody
- ARK1 antibody
see all
Images
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ab83968, at a dilution of 1/250, staining Aurora A in NBF fixed asynchronous HeLa cells by Immunocytochemistry/Immunofluorescence.
Detection: Anti-rabbit IgG-FITC conjugated antibody used at a dilution of 1/100. -
ab83968, at a dilution of 1/250, staining Aurora A in NBF fixed asynchronous HeLa cells by Immunocytochemistry/Immunofluorescence.
Mock phosphatase treated section (left), or calf intestinal phosphatase treated section (right).
Detection: Anti-rabbit IgG-FITC conjugated antibody used at a dilution of 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab83968 has been referenced in 5 publications.
- Biswas S et al. PEITC: A resounding molecule averts metastasis in breast cancer cells in vitro by regulating PKCδ/Aurora A interplay. Heliyon 8:e11656 (2022). PubMed: 36458309
- Kuriyama R & Fisher CR A novel mitosis-specific Cep215 domain interacts with Cep192 and phosphorylated Aurora A for organization of spindle poles. J Cell Sci 133:N/A (2020). PubMed: 33376154
- Kiseleva AA et al. Unexpected Activities in Regulating Ciliation Contribute to Off-target Effects of Targeted Drugs. Clin Cancer Res 25:4179-4193 (2019). PubMed: 30867219
- Blas-Rus N et al. Aurora A drives early signalling and vesicle dynamics during T-cell activation. Nat Commun 7:11389 (2016). PubMed: 27091106
- Whalley HJ et al. Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation. Nat Commun 6:7437 (2015). ICC/IF ; Human . PubMed: 26078008