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    aurora-b-antibody-ab70238.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Aurora
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Anti-Aurora B antibody (ab70238)

  • Datasheet
  • SDS
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Immunoprecipitation - Anti-Aurora B antibody (ab70238)
  • Western blot - Anti-Aurora B antibody (ab70238)
  • Immunocytochemistry - Anti-Aurora B antibody (ab70238)

Key features and details

  • Rabbit polyclonal to Aurora B
  • Suitable for: WB, IP, ICC
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Aurora B antibody
    See all Aurora B primary antibodies
  • Description

    Rabbit polyclonal to Aurora B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICCmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 300 and the C-terminus (residue 344) of Human Aurora B (Swiss-Prot Q96GD4).

  • Positive control

    • IP: Lysate from nocodazole treated HeLa cells; WB: Lysates from nocodazole treated HeLa cells; ICC: HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Aurora
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Phosphorylation
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Aurora
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Aurora

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Aurora B protein (ab51435)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab70238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000 - 1/15000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IP
Use at 2-10 µg/mg of lysate.
ICC
1/500 - 1/2000.
Notes
WB
1/5000 - 1/15000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IP
Use at 2-10 µg/mg of lysate.
ICC
1/500 - 1/2000.

Target

  • Function

    May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
  • Tissue specificity

    High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
  • Involvement in disease

    Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
  • Target information above from: UniProt accession Q96GD4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9212 Human
    • Omim: 604970 Human
    • SwissProt: Q96GD4 Human
    • Unigene: 442658 Human
    • Alternative names

      • AIK2 antibody
      • AIM-1 antibody
      • AIM1 antibody
      • ARK-2 antibody
      • ARK2 antibody
      • AurB antibody
      • AURKB antibody
      • AURKB_HUMAN antibody
      • Aurora 1 antibody
      • Aurora and Ipl1 like midbody associated protein 1 antibody
      • Aurora kinase B antibody
      • Aurora related kinase 2 antibody
      • Aurora- and Ipl1-like midbody-associated protein 1 antibody
      • Aurora-B antibody
      • Aurora-related kinase 2 antibody
      • Aurora/IPL1 related kinase 2 antibody
      • Aurora/IPL1-related kinase 2 antibody
      • IPL1 antibody
      • PPP1R48 antibody
      • Protein phosphatase 1 regulatory subunit 48 antibody
      • Serine/theronine kinase 12 antibody
      • Serine/threonine protein kinase 12 antibody
      • Serine/threonine-protein kinase 12 antibody
      • Serine/threonine-protein kinase aurora-B antibody
      • STK-1 antibody
      • STK1 antibody
      • STK12 antibody
      • STK5 antibody
      see all

    Images

    • Immunoprecipitation - Anti-Aurora B antibody (ab70238)
      Immunoprecipitation - Anti-Aurora B antibody (ab70238)

      Immunoprecipitation of nocodaze-treated HeLa whole cell lysate

      Lane 1: ab70238 current lot

      Lane 2: ab70238 previous lot

      Lane 3: Control IgG

    • Western blot - Anti-Aurora B antibody (ab70238)
      Western blot - Anti-Aurora B antibody (ab70238)
      All lanes : Anti-Aurora B antibody (ab70238) at 0.1 µg/ml

      Lane 1 : HeLa cells treated with Nocodazole at 50 µg
      Lane 2 : HeLa cells mock treated at 50 µg
      Lane 3 : HeLa cells treated with Nocodazole at 15 µg
      Lane 4 : HeLa cells mock treated at 15 µg
      Lane 5 : HeLa cells treated with Nocodazole at 5 µg
      Lane 6 : HeLa cells mock treated at 5 µg

      Predicted band size: 39 kDa


      Exposure time: 3 minutes
    • Immunocytochemistry - Anti-Aurora B antibody (ab70238)
      Immunocytochemistry - Anti-Aurora B antibody (ab70238)
      Immunocytochemistry/Immunofluorescence analysis of asynchronous HeLa cells labelling Aurora B with ab70238 at 0.5µg/ml in A and at 2µg/ml in B. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody in A. A Texas Red-conugated anti-rabbit IgG was used as the secondary antibody in B.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab70238? Please let us know so that we can cite the reference in this datasheet.

    ab70238 has been referenced in 3 publications.

    • Zhu Q  et al. Viral-Mediated AURKB Cleavage Promotes Cell Segregation and Tumorigenesis. Cell Rep 26:3657-3671.e5 (2019). PubMed: 30917319
    • Martz MK  et al. Leukemia-associated RhoGEF (LARG) is a novel RhoGEF in cytokinesis and required for the proper completion of abscission. Mol Biol Cell 24:2785-94 (2013). PubMed: 23885121
    • Wang E  et al. Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. J Cell Biol 194:539-49 (2011). ICC/IF ; Human . PubMed: 21844210

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