Anti-Aurora B antibody (ab70238)
Key features and details
- Rabbit polyclonal to Aurora B
- Suitable for: WB, IP, ICC
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Aurora B antibody
See all Aurora B primary antibodies -
Description
Rabbit polyclonal to Aurora B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
-
Immunogen
Synthetic peptide corresponding to a region between residue 300 and the C-terminus (residue 344) of Human Aurora B (Swiss-Prot Q96GD4).
-
Positive control
- IP: Lysate from nocodazole treated HeLa cells; WB: Lysates from nocodazole treated HeLa cells; ICC: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/15000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
|
|
| IP |
Use at 2-10 µg/mg of lysate.
|
|
| ICC |
1/500 - 1/2000.
|
| Notes |
|---|
|
WB
1/5000 - 1/15000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
|
IP
Use at 2-10 µg/mg of lysate. |
|
ICC
1/500 - 1/2000. |
Target
-
Function
May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP. -
Tissue specificity
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. -
Involvement in disease
Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsUbiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. -
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. - Information by UniProt
-
Database links
- Entrez Gene: 9212 Human
- Omim: 604970 Human
- SwissProt: Q96GD4 Human
- Unigene: 442658 Human
-
Alternative names
- AIK2 antibody
- AIM-1 antibody
- AIM1 antibody
see all
Images
-
Immunoprecipitation - Anti-Aurora B antibody (ab70238)
Immunoprecipitation of nocodaze-treated HeLa whole cell lysate
Lane 1: ab70238 current lot
Lane 2: ab70238 previous lot
Lane 3: Control IgG
-
Western blot - Anti-Aurora B antibody (ab70238)All lanes : Anti-Aurora B antibody (ab70238) at 0.1 µg/ml
Lane 1 : HeLa cells treated with Nocodazole at 50 µg
Lane 2 : HeLa cells mock treated at 50 µg
Lane 3 : HeLa cells treated with Nocodazole at 15 µg
Lane 4 : HeLa cells mock treated at 15 µg
Lane 5 : HeLa cells treated with Nocodazole at 5 µg
Lane 6 : HeLa cells mock treated at 5 µg
Predicted band size: 39 kDa
Exposure time: 3 minutes -
Immunocytochemistry - Anti-Aurora B antibody (ab70238)Immunocytochemistry/Immunofluorescence analysis of asynchronous HeLa cells labelling Aurora B with ab70238 at 0.5µg/ml in A and at 2µg/ml in B. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody in A. A Texas Red-conugated anti-rabbit IgG was used as the secondary antibody in B.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab70238 has been referenced in 3 publications.
- Zhu Q et al. Viral-Mediated AURKB Cleavage Promotes Cell Segregation and Tumorigenesis. Cell Rep 26:3657-3671.e5 (2019). PubMed: 30917319
- Martz MK et al. Leukemia-associated RhoGEF (LARG) is a novel RhoGEF in cytokinesis and required for the proper completion of abscission. Mol Biol Cell 24:2785-94 (2013). PubMed: 23885121
- Wang E et al. Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. J Cell Biol 194:539-49 (2011). ICC/IF ; Human . PubMed: 21844210