Anti-Aurora B antibody (ab70238)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-Aurora B antibody
See all Aurora B primary antibodies -
DescriptionRabbit polyclonal to Aurora B
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, ICC/IF, ICCmore details
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Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to a region between residue 300 and the C-terminus (residue 344) of Human Aurora B (Swiss-Prot Q96GD4).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab70238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/15000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). | |
| IP | Use at 1-4 µg/mg of lysate. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 21844210 | |
| ICC | 1/500 - 1/2000. |
Target
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FunctionMay be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
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Tissue specificityHigh level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
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Involvement in diseaseNote=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
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Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsUbiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. -
Cellular localizationNucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
- Information by UniProt
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Database links
- Entrez Gene: 9212 Human
- Omim: 604970 Human
- SwissProt: Q96GD4 Human
- Unigene: 442658 Human
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Alternative names
- AIK2 antibody
- AIM-1 antibody
- AIM1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab70238)Immunocytochemistry/Immunofluorescence analysis of asynchronous HeLa cells labelling Aurora B with ab70238 at 0.5µg/ml in A and at 2µg/ml in B. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody in A. A Texas Red-conugated anti-rabbit IgG was used as the secondary antibody in B. -
Western blot - Anti-Aurora B antibody (ab70238)All lanes : Anti-Aurora B antibody (ab70238) at 1/10000 dilution
Lanes 1 & 3 : Whole cell lysate from non-adherent HeLa cells treated with nocodazole (20h)
Lane 2 : Whole cell lysate from adherent HeLa cells treated with nocodazole (20h)
Lane 4 : Whole cell lysate from adherent HeLa cells treated with nocodazole (20h)
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 28 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Detection time: 10 secs (lanes 1,2) or 30 secs (lanes 3,4) -
Western blot - Anti-Aurora B antibody (ab70238)All lanes : Anti-Aurora B antibody (ab70238) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate from an asynchronous culture
Lane 2 : HeLa whole cell lysate treated with cycloheximide for 12 hours
Lane 3 : HeLa whole cell lysatetreated with cycloheximide for 24 hours
Lane 4 : HeLa whole cell lysate
treated with cycloheximide for 36 hours
Lane 5 : HeLa whole cell lysate treated with nocodazole for 20 hours
Lysates/proteins at 100 µg per lane.
Predicted band size: 39 kDa
Protocols
References
This product has been referenced in:
- Martz MK et al. Leukemia-associated RhoGEF (LARG) is a novel RhoGEF in cytokinesis and required for the proper completion of abscission. Mol Biol Cell 24:2785-94 (2013). Read more (PubMed: 23885121) »
- Wang E et al. Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. J Cell Biol 194:539-49 (2011). ICC/IF ; Human . Read more (PubMed: 21844210) »
