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    aurora-b-antibody-aurkb1521-ab218339.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Aurora
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Anti-Aurora B antibody [AURKB/1521] (ab218339)

  • Datasheet
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Western blot - Anti-Aurora B antibody [AURKB/1521] (ab218339)

    Key features and details

    • Mouse monoclonal [AURKB/1521] to Aurora B
    • Suitable for: WB
    • Reacts with: Human, Recombinant fragment
    • Isotype: IgG2b

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    Protein
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    Recombinant human Aurora B protein (ab51435)

    View more associated products

    Overview

    • Product name

      Anti-Aurora B antibody [AURKB/1521]
      See all Aurora B primary antibodies
    • Description

      Mouse monoclonal [AURKB/1521] to Aurora B
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human, Recombinant fragment
    • Immunogen

      Recombinant fragment within Human Aurora B aa 89-251. The exact sequence is proprietary.
      Database link: Q96GD4

    • Positive control

      • WB: Recombinant Aurora B protein; Human liver lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.2
      Preservative: 0.05% Sodium azide
      Constituents: 0.05% BSA, 99% PBS
    • Concentration information loading...
    • Purity

      Protein A/G purified
    • Clonality

      Monoclonal
    • Clone number

      AURKB/1521
    • Isotype

      IgG2b
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Kinases/Phosphatases
      • Aurora
      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Phosphorylation
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Aurora
      • Cancer
      • Cell cycle
      • Kinases/phosphatases
      • Aurora

    Associated products

    • Alternative Versions

      • Anti-Aurora B antibody [AURKB/1521] - BSA and Azide free (ab269754)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
    • Recombinant Protein

      • Recombinant human Aurora B protein (ab51435)
    • Related Products

      • Recombinant human Aurora B protein (ab51435)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab218339 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 39 kDa.
    Notes
    WB
    Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 39 kDa.

    Target

    • Function

      May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
    • Tissue specificity

      High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
    • Involvement in disease

      Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
    • Cellular localization

      Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
    • Target information above from: UniProt accession Q96GD4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9212 Human
      • Omim: 604970 Human
      • SwissProt: Q96GD4 Human
      • Unigene: 442658 Human
      • Alternative names

        • AIK2 antibody
        • AIM-1 antibody
        • AIM1 antibody
        • ARK-2 antibody
        • ARK2 antibody
        • AurB antibody
        • AURKB antibody
        • AURKB_HUMAN antibody
        • Aurora 1 antibody
        • Aurora and Ipl1 like midbody associated protein 1 antibody
        • Aurora kinase B antibody
        • Aurora related kinase 2 antibody
        • Aurora- and Ipl1-like midbody-associated protein 1 antibody
        • Aurora-B antibody
        • Aurora-related kinase 2 antibody
        • Aurora/IPL1 related kinase 2 antibody
        • Aurora/IPL1-related kinase 2 antibody
        • IPL1 antibody
        • PPP1R48 antibody
        • Protein phosphatase 1 regulatory subunit 48 antibody
        • Serine/theronine kinase 12 antibody
        • Serine/threonine protein kinase 12 antibody
        • Serine/threonine-protein kinase 12 antibody
        • Serine/threonine-protein kinase aurora-B antibody
        • STK-1 antibody
        • STK1 antibody
        • STK12 antibody
        • STK5 antibody
        see all

      Images

      • Western blot - Anti-Aurora B antibody [AURKB/1521] (ab218339)
        Western blot - Anti-Aurora B antibody [AURKB/1521] (ab218339)
        All lanes : Anti-Aurora B antibody [AURKB/1521] (ab218339) at 1 µg/ml

        Lane 1 : recombinant Aurora B protein
        Lane 2 : Human liver lysate

        Predicted band size: 39 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (1)

      Publishing research using ab218339? Please let us know so that we can cite the reference in this datasheet.

      ab218339 has been referenced in 1 publication.

      • Nie M  et al. AURKB promotes gastric cancer progression via activation of CCND1 expression. Aging (Albany NY) 12:1304-1321 (2020). PubMed: 31982864

      Customer reviews and Q&As

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