Recombinant
RabMAb

Recombinant Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

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Overview

  • Product name

    Anti-Aurora B antibody [EP1009Y] - BSA and Azide free
    See all Aurora B primary antibodies

  • Description

    Rabbit monoclonal [EP1009Y] to Aurora B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Aurora B aa 1-100 (N terminal). The exact sequence is proprietary.

  • General notes

    ab239837 is the carrier-free version of ab45145 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239837 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab239837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.

    • Tissue specificity

      High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.

    • Involvement in disease

      Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.

    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.

    • Cellular localization

      Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
    • Information by UniProt

    • Database links

      • Alternative names

        • AIK2 antibody
        • AIM-1 antibody
        • AIM1 antibody
        • ARK-2 antibody
        • ARK2 antibody
        • AurB antibody
        • AURKB antibody
        • AURKB_HUMAN antibody
        • Aurora 1 antibody
        • Aurora and Ipl1 like midbody associated protein 1 antibody
        • Aurora kinase B antibody
        • Aurora related kinase 2 antibody
        • Aurora- and Ipl1-like midbody-associated protein 1 antibody
        • Aurora-B antibody
        • Aurora-related kinase 2 antibody
        • Aurora/IPL1 related kinase 2 antibody
        • Aurora/IPL1-related kinase 2 antibody
        • IPL1 antibody
        • PPP1R48 antibody
        • Protein phosphatase 1 regulatory subunit 48 antibody
        • Serine/theronine kinase 12 antibody
        • Serine/threonine protein kinase 12 antibody
        • Serine/threonine-protein kinase 12 antibody
        • Serine/threonine-protein kinase aurora-B antibody
        • STK-1 antibody
        • STK1 antibody
        • STK12 antibody
        • STK5 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
        Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

        Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized  HeLa (Human epithelial cells from cervix adenocarcinoma cell line) cell lines labeling Aurora B with ab45145 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

        Confocal image showing midbody and centromeres staining on Hela cells

        The nuclear counterstain is DAPI (blue).

        Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

        The negative controls are as follows:-

        -ve control 1: ab45145 at 1/500 dilution followed by ab150120 at 1/1000 dilution.

        -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

        Ab45145 staining human Aurora B in human Hodgkin lymphoma by immunohistochemistry using paraffin embedded tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

        ab45145 showing positive staining in Normal tonsil tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

        ab45145 showing positive staining in Breast carcinoma tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

        ab45145 showing positive staining in Cervical carcinoma tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

        ab45145 showing positive staining in Urinary bladder transitional carcinoma tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

        ab45145 showing positive staining in Lung adenocarcinoma tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Other - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
        Other - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).

      References

      ab239837 has not yet been referenced specifically in any publications.

      Publishing research using ab239837? Please let us know so that we can cite the reference in this datasheet.

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