Name: Anti-Aurora B antibody [EP1009Y] - BSA and Azide free
Brand: RabMAb
SKU: ab239837

Product name

Anti-Aurora B antibody [EP1009Y] - BSA and Azide free
See all Aurora B primary antibodies
Description

Rabbit monoclonal [EP1009Y] to Aurora B - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IP, ICC/IF, IHC-P, WBmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human Aurora B aa 1-100 (N terminal). The exact sequence is proprietary.
General notes

The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF^®).
ab239837 is a PBS-only buffer format of ab45145. Please refer to ab45145 for recommended dilutions, protocols, and image data. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (K_D)

K_D = 5.50 x 10 ^-11 M
Learn more about K_D
Storage buffer

Constituent: PBS
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

EP1009Y
Isotype

IgG
Research areas

Alternative Versions
- Anti-Aurora B antibody [EP1009Y] (Alexa Fluor® 647) (ab197614)
- Anti-Aurora B antibody [EP1009Y] (ab45145)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Our Abpromise guarantee covers the use of ab239837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IP		Use at an assay dependent concentration.
ICC/IF		Use at an assay dependent concentration.
IHC-P		Use at an assay dependent concentration.
WB		Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.

Application notes

Is unsuitable for Flow Cyt.

Function

May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
Tissue specificity

High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Involvement in disease

Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications

Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
Cellular localization

Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
Target information above from: UniProt accession Q96GD4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 9212 Human
- Omim: 604970 Human
- SwissProt: Q96GD4 Human
- Unigene: 442658 Human
Alternative names
- AIK2 antibody
- AIM-1 antibody
- AIM1 antibody
- ARK-2 antibody
- ARK2 antibody
- AurB antibody
- AURKB antibody
- AURKB_HUMAN antibody
- Aurora 1 antibody
- Aurora and Ipl1 like midbody associated protein 1 antibody
- Aurora kinase B antibody
- Aurora related kinase 2 antibody
- Aurora- and Ipl1-like midbody-associated protein 1 antibody
- Aurora-B antibody
- Aurora-related kinase 2 antibody
- Aurora/IPL1 related kinase 2 antibody
- Aurora/IPL1-related kinase 2 antibody
- IPL1 antibody
- PPP1R48 antibody
- Protein phosphatase 1 regulatory subunit 48 antibody
- Serine/theronine kinase 12 antibody
- Serine/threonine protein kinase 12 antibody
- Serine/threonine-protein kinase 12 antibody
- Serine/threonine-protein kinase aurora-B antibody
- STK-1 antibody
- STK1 antibody
- STK12 antibody
- STK5 antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma cell line) cell lines labeling Aurora B with ab45145 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing midbody and centromeres staining on Hela cells

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows:-

-ve control 1: ab45145 at 1/500 dilution followed by ab150120 at 1/1000 dilution.

-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

Ab45145 staining human Aurora B in human Hodgkin lymphoma by immunohistochemistry using paraffin embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

ab45145 showing positive staining in Normal tonsil tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

ab45145 showing positive staining in Breast carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

ab45145 showing positive staining in Cervical carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

ab45145 showing positive staining in Urinary bladder transitional carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

ab45145 showing positive staining in Lung adenocarcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).
Other - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).

Datasheet

ab239837 has not yet been referenced specifically in any publications.

Publishing research using ab239837? Please let us know so that we can cite the reference in this datasheet.

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Recombinant Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

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