RecombinantRabMAb

Recombinant Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

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Flow Cytometry - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
  • Immunocytochemistry - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
  • Immunoprecipitation - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
  • OI-RD Scanning - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
  • Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1009Y] to Aurora B - BSA and Azide free
  • Suitable for: ICC, IP, IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Aurora B antibody [EP1009Y] - BSA and Azide free
    See all Aurora B primary antibodies

  • Description

    Rabbit monoclonal [EP1009Y] to Aurora B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HeLa whole cell lysate.

  • General notes

    ab239837 is the carrier-free version of ab45145 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239837 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab239837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.

Target

  • Function

    May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.

  • Tissue specificity

    High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.

  • Involvement in disease

    Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.

  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
  • Information by UniProt

  • Database links

    • Alternative names

      • AIK2 antibody
      • AIM-1 antibody
      • AIM1 antibody
      • ARK-2 antibody
      • ARK2 antibody
      • AurB antibody
      • AURKB antibody
      • AURKB_HUMAN antibody
      • Aurora 1 antibody
      • Aurora and Ipl1 like midbody associated protein 1 antibody
      • Aurora kinase B antibody
      • Aurora related kinase 2 antibody
      • Aurora- and Ipl1-like midbody-associated protein 1 antibody
      • Aurora-B antibody
      • Aurora-related kinase 2 antibody
      • Aurora/IPL1 related kinase 2 antibody
      • Aurora/IPL1-related kinase 2 antibody
      • IPL1 antibody
      • PPP1R48 antibody
      • Protein phosphatase 1 regulatory subunit 48 antibody
      • Serine/theronine kinase 12 antibody
      • Serine/threonine protein kinase 12 antibody
      • Serine/threonine-protein kinase 12 antibody
      • Serine/threonine-protein kinase aurora-B antibody
      • STK-1 antibody
      • STK1 antibody
      • STK12 antibody
      • STK5 antibody
      see all

    Images

    • Flow Cytometry - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
      Flow Cytometry - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
      This data was developed using ab45145, the same antibody clone in a different buffer formulation.
      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Aurora B with Purified ab45145 at 1:300 dilution (0.1 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor ® 488 ,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Left). Unlabeled control - /.
    • Immunocytochemistry - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
      Immunocytochemistry - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

      This data was developed using ab45145, the same antibody clone in a different buffer formulation.
      Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Aurora B with Purified ab45145 at 1:50 dilution (5.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunoprecipitation - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
      Immunoprecipitation - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

      This data was developed using ab45145, the same antibody clone in a different buffer formulation.
      Purified ab45145 at 1/20 dilution (1µg) immunoprecipitating Aurora B in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab45145 + HeLa whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45145 in HeLa whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 39 kDa

    • OI-RD Scanning - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
      OI-RD Scanning - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).

    • Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
      Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab239837? Please let us know so that we can cite the reference in this datasheet.

    ab239837 has not yet been referenced specifically in any publications.

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