Western blot abreview for Anti-Aurora B antibody [mAbcam 3609]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293# HeLa(+)# NIH-3T3# PC12#9L)
Loading amount
30 µg
Specification
HEK293# HeLa(+)# NIH-3T3# PC12#9L
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Other product details

Concentration
2 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA, PBS-Tween-20 (0.1%)

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
Supersignal west dura
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 39 kDa Non-specific: 60 (PC12) lysate onl kDa
Positive control
Hela

Additional data

Additional Notes
Lane order (1-5): HEK293# HeLa(+)# NIH-3T3# PC12#9L
A species of the predicted mobility is observed in the positive control lysate (Hela) and also HEK293, PC12 and 9L suggesting that this antibody recognises rodent aurora B. This antibody performs better with BSA as the non-specific block (as opposed to milk).

Mr. Antibody Solutions

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Submitted Aug 26 2008

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