Immunocytochemistry/ Immunofluorescence abreview for Anti-Aurora B antibody [mAbcam 3609]

Inconclusive
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (3T3-L1 fibroblast)
Permeabilization
Yes - Triton X100
Specification
3T3-L1 fibroblast
Blocking step
0.1% donkey serum and 0.2% fish skin gelatin as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 20°C
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 0.1% serum and 0.2% fish skin gelatin
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-mouse. Alexa flour 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Undifferentiated 3T3-L1 fibroblasts were fixed with PFA for one hour followed by permeabilization and blocking in serum and fish skin gelatin. Cells were stained for aurora B and for tubulin.

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Submitted Dec 06 2016

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