Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aurora B antibody [mAbcam 3609]

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Permeabilization
Yes - 0.025% Triton X-100
Specification
Heart
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% serum
Dilution
1/200

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Invitrogen Goat anti-Mouse IgG (H+L) Cross-Adsorbe
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
No signal was found in paraffin embedded mouse cardiac tissue. Cardiac tissues were derived from day 6 or day 21 mouse.
Abcam response
Abcam has not tested ab3609 in Mouse samples and thus cannot say how it should have performed. This individual received a credit for testing the antibody in Mouse samples as part of our AbTrial program.

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Submitted Feb 05 2019

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