Recombinant
RabMAb

Recombinant Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706)

Rabbit recombinant monoclonal Aurora B (phospho S227) antibody [EPR20389]. Validated in WB, IP, Dot, ICC/IF and tested in Human. Independently reviewed in 1 review(s).

Overview

  • Product name
    Anti-Aurora B (phospho S227) antibody [EPR20389]
    See all Aurora B primary antibodies
  • Description
    Rabbit monoclonal [EPR20389] to Aurora B (phospho S227)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Dot blot, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Aurora B aa 200-300 (phospho S227) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96GD4

  • Positive control
    • WB: HeLa treated with 100 ng/ml nocodazole for 18 hours, whole cell lysate. ICC/IF: HeLa cells. IP: HeLa treated with 100 ng/ml nocodazole for 18 hours, whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab210706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
Dot blot 1/1000.
ICC/IF 1/10000.
IP 1/30.

Target

  • Function
    May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
  • Tissue specificity
    High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
  • Involvement in disease
    Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
  • Cellular localization
    Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIK2 antibody
    • AIM-1 antibody
    • AIM1 antibody
    • ARK-2 antibody
    • ARK2 antibody
    • AurB antibody
    • AURKB antibody
    • AURKB_HUMAN antibody
    • Aurora 1 antibody
    • Aurora and Ipl1 like midbody associated protein 1 antibody
    • Aurora kinase B antibody
    • Aurora related kinase 2 antibody
    • Aurora- and Ipl1-like midbody-associated protein 1 antibody
    • Aurora-B antibody
    • Aurora-related kinase 2 antibody
    • Aurora/IPL1 related kinase 2 antibody
    • Aurora/IPL1-related kinase 2 antibody
    • IPL1 antibody
    • PPP1R48 antibody
    • Protein phosphatase 1 regulatory subunit 48 antibody
    • Serine/theronine kinase 12 antibody
    • Serine/threonine protein kinase 12 antibody
    • Serine/threonine-protein kinase 12 antibody
    • Serine/threonine-protein kinase aurora-B antibody
    • STK-1 antibody
    • STK1 antibody
    • STK12 antibody
    • STK5 antibody
    see all

Images

  • All lanes : Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706) at 1/1000 dilution

    Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa treated with 100 ng/ml nocodazole for 18 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Aurora B (phospho S227) with ab210706 at 1/10000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing midbody staining (arrow) on HeLa cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Aurora B (phospho S227) was immunoprecipitated from 0.35 mg of HeLa (human  epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole for 18 hours whole cell lysate with ab210706 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab210706 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa treated with 100 ng/ml nocodazole for 18 hours whole cell lysate 10μg (Input).

    Lane 2: ab210706 IP in HeLa treated with 100 ng/ml nocodazole for 18 hours whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab210706 in HeLa treated with 100 ng/ml nocodazole for 18 hours whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds

  • Dot blot analysis of Aurora B (phospho S227) labeled with ab210706 at 1/1000 dilution.

    Lane 1: Aurora B (phospho S227) peptide.

    Lane 2: Aurora B non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes 

References

ab210706 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Filter by Application

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa/DLD1)
Permeabilization
Yes - 0.5% Tx-100, 5min
Specification
HeLa/DLD1
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Tanya Soliman

Verified customer

Submitted Oct 07 2017

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