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  1. Link

    aurora-b-phospho-t232-antibody-ab115793.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Aurora
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Anti-Aurora B (phospho T232) antibody (ab115793)

  • Datasheet
Reviews (3) Submit a question References (4)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B (phospho T232) antibody (ab115793)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B (phospho T232) antibody (ab115793)

Key features and details

  • Rabbit polyclonal to Aurora B (phospho T232)
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Aurora B (phospho T232) antibody
    See all Aurora B primary antibodies
  • Description

    Rabbit polyclonal to Aurora B (phospho T232)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Aurora B (phospho T232).
    Database link: Q96GD4

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Aurora
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Phosphorylation
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Aurora
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Aurora

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Aurora B protein (ab51435)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab115793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml.
Notes
IHC-P
Use a concentration of 5 µg/ml.

Target

  • Function

    May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
  • Tissue specificity

    High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
  • Involvement in disease

    Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
  • Target information above from: UniProt accession Q96GD4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9212 Human
    • Omim: 604970 Human
    • SwissProt: Q96GD4 Human
    • Unigene: 442658 Human
    • Alternative names

      • AIK2 antibody
      • AIM-1 antibody
      • AIM1 antibody
      • ARK-2 antibody
      • ARK2 antibody
      • AurB antibody
      • AURKB antibody
      • AURKB_HUMAN antibody
      • Aurora 1 antibody
      • Aurora and Ipl1 like midbody associated protein 1 antibody
      • Aurora kinase B antibody
      • Aurora related kinase 2 antibody
      • Aurora- and Ipl1-like midbody-associated protein 1 antibody
      • Aurora-B antibody
      • Aurora-related kinase 2 antibody
      • Aurora/IPL1 related kinase 2 antibody
      • Aurora/IPL1-related kinase 2 antibody
      • IPL1 antibody
      • PPP1R48 antibody
      • Protein phosphatase 1 regulatory subunit 48 antibody
      • Serine/theronine kinase 12 antibody
      • Serine/threonine protein kinase 12 antibody
      • Serine/threonine-protein kinase 12 antibody
      • Serine/threonine-protein kinase aurora-B antibody
      • STK-1 antibody
      • STK1 antibody
      • STK12 antibody
      • STK5 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B (phospho T232) antibody (ab115793)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B (phospho T232) antibody (ab115793)

      Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Aurora B (phospho T232) with ab115793 at 5 ug/ml.

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B (phospho T232) antibody (ab115793)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B (phospho T232) antibody (ab115793)

      Immunohistochemical analysis of paraffin embedded Human adrenal tissue labeling Aurora B (phospho T232) with ab115793 at 5 ug/ml.

       

       

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (4)

    Publishing research using ab115793? Please let us know so that we can cite the reference in this datasheet.

    ab115793 has been referenced in 4 publications.

    • Li M  et al. Targeting Aurora B kinase with Tanshinone IIA suppresses tumor growth and overcomes radioresistance. Cell Death Dis 12:152 (2021). PubMed: 33542222
    • Shaalan AK  et al. Inhibition of Aurora Kinase B activity disrupts development and differentiation of salivary glands. Cell Death Discov 7:16 (2021). PubMed: 33462217
    • Yin H  et al. Chiauranib selectively inhibits colorectal cancer with KRAS wild-type by modulation of ROS through activating the p53 signaling pathway. Am J Cancer Res 10:3666-3685 (2020). PubMed: 33294260
    • Deng X  et al. PRMT1 promotes mitosis of cancer cells through arginine methylation of INCENP. Oncotarget 6:35173-82 (2015). PubMed: 26460953

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Aurora B (phospho T232) antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Sample
    Mouse Cell (NMuMG)
    Specification
    NMuMG
    Permeabilization
    Yes - PBS Tx100 0,3%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 20 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aurora B (phospho T232) antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample
    Mouse Tissue sections (Mouse 14.5dpc)
    Specification
    Mouse 14.5dpc
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 15 2013

    Western blot abreview for Anti-Aurora B (phospho T232) antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (15% acrilamide)
    Sample
    Mouse Cell lysate - whole cell (NmMG)
    Specification
    NmMG
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 01 2013

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