Overview

  • Product name

  • Description

    Rabbit polyclonal to AUTS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human AUTS2 aa 1009-1114.
    Sequence:

    NSSSSVHPGPLASMPMTVGVTGIHPMNSISSLDRTRMMTPFMGISPLPGG ERFPYPSFHWDPIRDPLRDPYRELDIHRRDPLGRDFLLRNDPLHRLSTPR LYEADR


    Database link: Q8WXX7

  • Positive control

    • IHC: Human cerebral cortex, skin and prostate tissue. ICC/IF: A431 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Strongly expressed in brain, skeletal muscle and kidney. Also expressed in placenta, lung and leukocytes.
  • Sequence similarities

    Belongs to the AUTS2 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Autism related protein 1 antibody
    • Autism susceptibility candidate 2 antibody
    • Autism susceptibility gene 2 protein antibody
    • AUTS2 antibody
    • AUTS2_HUMAN antibody
    • FBRSL2 antibody
    • KIAA0442 antibody
    • MGC13140 antibody
    see all

Images

  • Paraffin-embedded human cerebral tissue stained for AUTS2 using ab224035 at 1/200 dilution in immunohistochemical analysis.

  • Paraffin-embedded human skin tissue stained for AUTS2 using ab224035 at 1/200 dilution in immunohistochemical analysis.

  • Paraffin-embedded human prostate tissue stained for AUTS2 using ab224035 at 1/200 dilution in immunohistochemical analysis.

  • Paraffin-embedded human skeletal muscle tissue stained for AUTS2 using ab224035 at 1/200 dilution in immunohistochemical analysis (negative control).

  • PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for AUTS2 (green) using ab224035 at 4 µg/ml in ICC/IF.

References

ab224035 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab224035.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up