• Product name
  • Description
    Rabbit polyclonal to AUTS2
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 524 and 770 of AUTS2

  • Positive control
    • 293T and NIH-3T3 cell lysate


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab96326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 139 kDa.



  • Anti-AUTS2 antibody (ab96326) at 1/1000 dilution + 293T cell lysate at 30 µg

    Predicted band size: 139 kDa

  • Anti-AUTS2 antibody (ab96326) at 1/1000 dilution + NIH3T3 cell lysate at 30 µg

    Predicted band size: 139 kDa


This product has been referenced in:
  • Oksenberg N  et al. Genome-wide distribution of Auts2 binding localizes with active neurodevelopmental genes. Transl Psychiatry 4:e431 (2014). ChIP ; Mouse . Read more (PubMed: 25180570) »

See 1 Publication for this product

Customer reviews and Q&As

Unfortunately this is the only buffer formulation available for this product at present. You may remove the glycerol by use of an Antibody Concentration Kit (ab102776). Dilute the antibody to reduce the glycerol’s concentration, which in turn will mak...

Read More

Thank you for your reply and for providing the protocol information.

Your protocol looks ok to me and so should be fine.

I just had a few other questions, have you done a no primary control? Also now that I look at your blot again, ...

Read More

Thank you for contacting Abcam.

The antibody, ab25788, is covered under our Abpromise for six months and is guaranteed to work in western blot on mouse and human samples. We have not yet tested the antibody in IHC and therefore it may not be ...

Read More

Thank you for that information.

Can I just clarify something. You mentioned that the gel was probed twice, onceusing 1% milk in 1x PBS 0.3%Tween.This was then restained using5% milk in 1xTBS 0.05%Tween. For the first condition did you s...

Read More

Thank you for calling us and for alerting us to the problem you are experiencing with Anti-AUTS2 antibodyab96326. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we di...

Read More

Thank you for contacting us. The immunogen that was used to create ab96326 shows 100% identity with human long isoform of AUTS2 and 89% identity with short isoform of AUTS2. So this antibody will be detecting both the isoforms. I hope this info...

Read More

Thank you for contacting us. I have read the whole protocol you have kindly submitted. In order to address the problem quickly I have further questions to ask. - According to the attached image you have used non transfected N2a mouse cells, Lym...

Read More

Thank you for your email. I can confirm that I added the discount code to our internal documents of the Abreview 26773. Your customer can now redeem this discount code and order a free antibody. Please thank your customer for he...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Cell lysate - nuclear (N2a mouse neuroblastoma cells)
Loading amount
150000 cells
N2a mouse neuroblastoma cells
lysed in radioimmunoprecipitation (RIPA) buffer (150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 50 mM Tris-HCl, pH 7.5, 2 mM EDTA) supplemented with protease inhibitor cocktail for 20 min. on ice
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Dr. Laura Malki Feldman

Verified customer

Submitted Nov 03 2011

Thank you for your recent enquiry which has been forwarded to me as my colleague is currently away from the office. I can confirm that ab96326 Anti-AUTS2 antibody was used to detect endogenous AUTS2 in 293T and NIH3T3 cells in the images on the da...

Read More


Sign up