Anti-AUTS2 antibody [CL7067] (ab243042)
Key features and details
- Mouse monoclonal [CL7067] to AUTS2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-AUTS2 antibody [CL7067]
See all AUTS2 primary antibodies -
Description
Mouse monoclonal [CL7067] to AUTS2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human AUTS2 aa 1050-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8WXX7 -
Positive control
- IHC-P: Mouse embryo E14 and cerebellum tissue. ICC/IF: U-251 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
CL7067 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 2 - 10 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 2 - 10 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Strongly expressed in brain, skeletal muscle and kidney. Also expressed in placenta, lung and leukocytes. -
Sequence similarities
Belongs to the AUTS2 family. - Information by UniProt
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Database links
- Entrez Gene: 26053 Human
- Entrez Gene: 319974 Mouse
- Omim: 607270 Human
- SwissProt: Q8WXX7 Human
- SwissProt: Q8VDM3 Mouse
- Unigene: 21631 Human
- Unigene: 712991 Human
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Alternative names
- Autism related protein 1 antibody
- Autism susceptibility candidate 2 antibody
- Autism susceptibility gene 2 protein antibody
see all
Images
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Paraffin-embedded mouse embryo E14 tissue stained for AUTS2 using ab243042 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells labeling AUTS2 (green) using ab243042 at 10 µg/mL in ICC/IF analysis. Microtubules visulized in red.
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Paraffin-embedded mouse cerebellum tissue stained for AUTS2 using ab243042 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse embryo E14 tissue stained for AUTS2 using ab243042 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab243042 has not yet been referenced specifically in any publications.